YAP65

GWB-487991

A synthetic peptide corresponding to residues near the C-terminus of human YAP65 was used as an immunogen.

WB: 1:1 000 5 000IHC: 1:100 - 250ICC: 1:100 - 250FC: 1:25IP: 1:70. The transcriptional coactivator Yes-associated protein (YAP) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). Akt promotes YAP localization to the cytoplasm resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases while YAP depletion decreases p73-mediated apoptosis following DNA damage in an Akt inhibitable manner (2) 1. Strano S et al. Mol Cell 18(4):447-59 20052. Basu S et al. Mol Cell 11(1):11-23 20033. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487