LEF-1/TCF-1 alpha

GWB-B0EE29

A synthetic peptide corresponding to residues in human LEF-1/TCF-1 alpha was used as an immunogen.

WB: 1:1 000 - 2 000IHC: 1:50ICC: 1:100 - 250FC: 1:20. The human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5\' -PyCTTTG-3\' ) present in a variety of other T cell-specific control regions. TCF-1 alpha is currently unique among the newly emerging family of DNA-binding regulatory proteins that share the HMG motif in that it is a highly tissue-specific RNA polymerase II transcription factor (1). TCF-1 alpha participates in the regulation of TCR alpha enhancer by facilitating the assembly of multiple proteins into a higher order nucleoprotein complex. The function of LEF-1 is dependent in part on the HMG domain that induces a sharp bend in the DNA helix and on an activation domain that stimulates transcription only in a specific context of other enhancer-binding proteins (2). Constitutive activation of the Wnt signaling pathway is a root cause of many colon cancers. Activation of this pathway is caused by genetic mutations that stabilize the beta-catenin protein allowing it to accumulate in the nucleus and form complexes with any member of the lymphoid enhancer factor and T-cell factor family of transcription factors (referred to collectively as LEF/TCFs) to activate transcription of target genes (3). 1. Waterman ML et al. Genes Dev 5(4):656-69 19912. Bruhn L et al. Genes Dev 11(5):640-53 19973. Hovanes K et al. Nat Genet 28(1):53-7 20014. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487