NF-kappa-B p65 Phospho (pS536)

GWB-5DAAAA

A synthetic phospho-peptide corresponding to residues surrounding serine 536 of human NF-kappa-B protein.

WB: 1:10 000-20 000IP: 1:20. Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50 p52 p65 (RelA) c-Rel and RelB. (2). The p65 subunit similar to two others in the B family RelB and c-Rel contains two transactivation domains in the C-terminal region of the protein (3). Association with inhibitory proteins of the IB family retains NFB in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS) leading to nuclear translocation and subsequent binding of NF-B to DNA (4). p65 phosphorylation events occur in the cytoplasm or in the nucleus and are stimuli-specific and cell-type specific on serine 276 529 and 536 by various kinases such as PKA casein kinase II IKK beta and RSK1. Phosphorylation on these sites has been linked to regulation of NFB activation (5). 1. Baldwin A. S. Jr. (1996) Annu. Rev. Immunol. 14 649-6832. Verma I. Stevenson J. Schwarz E. Van Antwerp D. & Miyamoto S. (1995) Genes Dev. 9 2723-27353. Schmitz M. dos Santos Silva M. & Baeuerle P. (1995) J. Biol. Chem. 270 15576-155844. Marc D. Jacobs Stephen C. Harrison (1198) Cell. Volume 95 Issue 6 749-7585. Wang Y et al. Br J Pharmacol. 2006 July; 148(6): 8146. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487