p53 Phospho (pS33)

GWB-52B5E8

A phospho specific peptide corresponding to residues surrounding serine 33 of human p53 was used as an immunogen. This antibody detects p53 phosphorlyated at serine 53.

WB: 1:1000ICC: 1:100. p53 acts as both a tumor-suppressor and transcription factor that upon activation by DNA damage and other cellular stress signals leads to the transcription of genes triggering cell-cycle arrest apoptosis and DNA repair (1 2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers (4 5). Cyclin dependent kinase 9 whose well-known substrate is RNA polymerase II can also phosphorylate p53 more specifically Ser33 on the N-terminus and Ser315 and Ser392 on the C-terminus (6). 1. Levine A. J. et al. Cell 88: 3232. Lakin N. D. et al. Oncogene 18: 76443. Vogelstein B. et al. Nature 348: 6814. Hoolstein M. et al. Science 253: 495. Harris C. C. Science 262: 19806. Radhakrishnan SK et al. Cell Cycle 5(5):519-21 20067. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487