FADD Phospho (pS194)

GWB-C281F7

A synthetic peptide corresponding to residues surrounding serine 194 of human FADD was used as immunogen.

WB: 1:500IP: 1:30. Fas-associating protein with death domain (FADD) is an adaptor protein involved in the apoptosis induced by Fas and TNF-R1 and mitogen induced cell proliferation (1). FADD consists of death domain (DD) at the C-terminal and death effector domain (DED) at the N-terminal (2). DD interacts with Fas and TNF-R1 which exposes the DED portion on the FADD. Unmasked DED binds to the DED of pro-caspase-8 which activates the cysteine protease cascade (3). The development of autoimmune lymphoproliferative (lpr) diseases has been linked to inactivation of Fas-FADD interaction (4). Also the deficiency in FADD can inhibit TRAIL-induced cell apoptosis. In cancer cells higher phopsholyation of FADD at serine 194 is expressed compared to normal cells. Phospholyation at serine 194 has been linked to induction of apoptosis by anti-cancer drugs in human protstate cancer cells and it is considered as a valuable maker for human prostate cancer progression (5). 1. Imtiyaz HZ et al I J. Biol. Chem. 280(36); 31360-67 20052. Chinnaiyan AM et al. Cell 81 5053. Boldin MP et al Cell 85 8034. Cohen PL et al. Annu. Rev. Immunol. 9 2435. Shimada K et al. J Pathol 206:423-32 2005. 6. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487