PKC delta Phospho (pY311)

GWB-C42F57

A phospho-specific peptide corresponding to residues surrounding Tyrosine 311 of human PKC-delta was used as an immunogen. The antibody only detects PKC-delta phosphorylated on Tyrosine 311.

WB: 1:2500 - 5000IHC: 1:100IP: 1:40. PKC-delta has a biological activity which is closely related to Pkc1. PKC-delta activates the Pkc1-mediated pathway through an activation of the Bck1 kinase. PKC-delta appears to play a critical role in growth control of yeast and mammalian cells. Suppression experiments suggest that PKC-delta desensitizes the pathway by regulating an aspect of G protein function (1). PKC-delta phosphorylates hRad9 in vitro and in cells exposed to genotoxic agents. It has been shown that PKC-delta is required for binding of hRad9 to Bcl-2. In concert with these results inhibition of PKC-delta attenuates Rad9-mediated apoptosis. These findings demonstrate that PKC-delta is responsible for the regulation of Rad9 in the Hus1-Rad1 complex and in the apoptotic response to DNA damage (2). PKC-delta is phosphorylated on tyrosine residues in response to thrombin and collagen and it has recently been shown that Tyrosine 311 is phosphorylated in response to protease-activated receptor 1 and protease-activated receptor 4 activation (3). 1. Nomoto S et al. Genes Cells 2(10):601-14 19972. Yoshida K et al. EMBO J 22(6):1431-41 20033. HALL K J et al. Biochem. J. 406 (501-509) 20074. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487