D-Dimer [DD6]

DD6

This clone has been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot.

ab10056 is specific for D-dimer and high molecular weight fibrin degradation products. Low cross-reaction with D-monomer cross-reaction with fibrinogen. Good for detection MAb in two-step sandwich IA.

Purity is tested by electrophoresis. Storage

ELISA: Use at an assay dependent dilution. IA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Can be paired for ELISA with 20-272-190038 Mouse monoclonal [DD1] to D-Dimer and 20-272-190040 Mouse monoclonal [DD2] to D-Dimer . To be analyzed in sandwich immunoassay plasma must be diluted with 10 mM Tris/HCl buffer pH 7. 5 containing 1 M NaCl and 0. 1 % Tween 20. To avoid non-specific binding the final NaCl concentration in plasma samples must be 0. 5 M or more. Less than 10 ng/ml of D-Dimer can be detected by DD1-DD6 assay using ordinary ELISA approach with HRP-labeled DD6 MAb. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Myeloma: Sp2/0 Fibrinogen is the main protein of blood coagulation system. It consists of two identical subunits that contain three polypeptide chains: alpha beta and gamma. The process of blood coagulation results in the activation of fibrinogen into fibrin by thrombin and fibrin polymerization. Fibrin clot is then digested by plasmin and fibrin degradation products of different molecular weights are released into the bloodstream.