Eotaxin [42285.111]

GWB-119268

IgG2a

Mouse

Eotaxin

0. 5 mg/ml

NoneConstituents: PBS

8. 4 kDa. The detection limit for recombinant mouse eotaxin is approximately 20 ng/lane under non-reducing and reducing conditions. Monoclonal Eotaxin has the ability to neutralize the biological activity of recombinant mouse eotaxin. It will also neutralize the biological activity of human eotaxin under similar conditions. To measure this chemoattractant activity recombinant mouse eotaxin is incubated with various concentrations of the antibody for 30 minutes at room temperature in a 96 well microplate. Following this preincubation period 35 µ l of the cytokine-antibody solution (containing recombinant mouse eotaxin at a final concentration of 0. 1 µ g/ml and antibody at concentrations from 0. 01 to 100 µ g/ml) is transferred to the lower compartment of a 96 well chemotaxis chamber. The chemotaxis chamber is then assembled using a PVP-free polycarbonate filter (5 micron pore size) and 2 x 10^6 cells/ well (CCR-3 transfected Y3 rat myeloid cells) are added to the top chamber. After incubation for 3 hours at 37 C in a 5 % CO2 humidified incubator the chamber is disassembled and the cells that have migrated through to the lower chamber are transferred to a working plate and stained using MTT. Absorbance is read on a microplate reader. The exact concentration of antibody required to neutralize recombinant mouse eotaxin activity is dependent on the cytokine concentration cell type growth conditions and the type of activity. The Neutralization Dose50 (ND50) for the antibody is defined as that concentration required to yield one-half maximal inhibition of the eotaxin activity on a responsive cell line when eotaxin is present at a concentration just high enough to elicit a maximum response. The Neutralization Dose50 (ND50) for this antibody is approximately 10 to 30 µ g/ml in the presence of 100 ng/ml of recombinant mouse eotaxin measuring the chemotaxis of CCR-3 transfected rat myeloid Y3 cellsNot tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular

In response to the presence of allergens this protein directly promotes the accumulation of eosinophils a prominent feature of allergic inflammatory reactions. Binds to CCR3.

By TNF-alpha interleukin-1 alpha and interferon gamma.

Belongs to the intercrine beta (chemokine CC) family.