Nucleophosmin [FC82291]

GWB-23B132

IgG1

Rat

NIH3T3 whole cell lysate

Nuclear. Generally nucleolar but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs.

15mM Sodium Azide; Constituents: 1% BSA 0. 01M PBS. pH 7. 4

Nuclear. Generally nucleolar but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. B23 (also known as NPM nucleophosmin numatrin or NO38) is a phosphoprotein widely expressed in all cell types and localized in granular regions of the nucleolus associated with pre-ribosomal particles. This protein was found to be involved in several nuclear functions such as assembly and/or intranuclear transport of preribosomal particles cytoplasmic/nuclear trafficking regulation of DNA polymerase alpha activity and centrosome duplication. Phosphorylation of B23 is mediated by a cyclin complex CDK2/cyclin E. This phosphorylation causes the dissociation of B23 from the centrosome and enables the duplication of the centrosome during cell mitosis. B23 was also found to be involved in pro-myelocytic leukemia in which the gene for B23 fuses with retinoic acid receptor alpha (RAR alpha). As a consequence a fusion protein (60-70 kDa) is produced containing parts of the two genes. Monoclonal antibody reacting specifically with B23 is a useful tool for the study of the involvement of B23 in nuclear functions cytoplasmic/nuclear trafficking and centrosome duplication.

Disulfide-linked dimers under certain conditions. The SWAP complex consists of NPM1 NCL PARP1 and SWAP70 (By similarity). Interacts with NSUN2.

Phosphorylated.

A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5; 17)(q32; q11) with RARA.

Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.