SNAP25 [SP12]

SP12

Crude synaptic immunoprecipitate (Human).

Brain

Cytoplasmic and Plasma membrane

0. 09% Sodium Azide; Constituents: PBS. pH 7. 2

Cytoplasmic and Plasma membrane Synaptic vesicle membrane docking and fusion is mediated by SNAREs(soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t- NAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.

Part of the SNARE core complex containing SNAP25 VAMP2 and STX1A. This complex binds CPLX1. Interacts with TRIM9 RIMS1 SNAP25BP and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1 SV2B and syntaxin-1 (By similarity).

Neurons of the neocortex hippocampus piriform cortex anterior thalamic nuclei pontine nuclei and granule cells of the cerebellum.

When cloned and expressed in Eschericia coli where protein palmitoylation does not occur Cys-85 Cys-88 Cys-90 and Cys-92 in the protein sequence readily form an iron-sulfur cluster instead.

Contains 2 t-SNARE coiled-coil homology domains.