LEF1 [3A12]

3A12

Synthetic peptide: DTSMSRF corresponding to amino acids 236-242 of Human LEF1.

Reacts with LEF1 Transactivation Domain.

Nuclear

The antibody is purified from ascites fluid using Protein G chromatography. Storage

WB: Use at a concentration of 1 - 5 µ g/ml. Predicted MW 55-57 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1 TLE2 TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.

Nucleus (By similarity). Note=Found in nuclear bodies upon PIASG binding (By similarity).

Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.

Contains 1 HMG box DNA-binding domain.