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NCOA3 [AX 15]

NCOA3 [AX 15]

Item no.	20-272-190709
Manufacturer	GENWAY
Amount	0.05 ml
Category	Oncology Neuroscience Targets for Rodent Neurosciences Antibodies By Cell Type Hematology Leukemia Lymphoma Cell Biology Cell Movement Cell Adhesion Immunoprecipitation Western Blot Primary Antibodies By Organ Breast Cancer
Type	Antibody
Applications	WB, IP
Clone	AX 15
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-297821
Similar products	20-272-190709
Available
Genway ID:	GWB-297821
Clone:	AX 15
Isotype:	IgG
Immunogen:	Fusion protein containing amino acids 605-1294 of human NCOA3.
Antigen Species:	Human
Positive Control:	BG-1 ovarian cell RIPA lysate
Target:	NCOA3
Localization:	Mainly cytoplasmic and weakly nuclear Storage
Preservative:	0. 035% Sodium Azide; Constituents: 30% Glycerol Raw Ascites
Application Note:	IP: 15µ l of 20-272-190709 will immunoprecipitate NCOA3 from 0. 5-1 mg of a BG-1 ovarian cell RIPA lysate. WB: Use at a dilution of 1/500. 20-272-190709 recognizes NCOA3 (150 kD) and another doublet (110 kD) by Western blotting a BG-1 ovarian cell RIPA lysate. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular
Localization:	Mainly cytoplasmic and weakly nuclear NCOA3 is overexpressed in breast and ovarian cancer cell lines. The SRC-1 family of coactivators of which NCOA3 is a member interacts with steroid hormones and enhances transcription. NCOA3 interacts with estrogen receptors in breast and ovarian cancer cell lines and induces extra transcription. NCOA3 thus contributes to the development of breast and ovarian cancer when it interacts with estrogen receptors in this way. In addition to NCOA3\' s cancer involvement it also plays a role in the formation of a multisubunit coactivator complex by gathering together the two nuclear factors CBP and P/CAF.
Function:	Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors such as for steroids (GR and ER) retinoids (RARs and RXRs) thyroid hormone (TRs) vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
Catalytic Activity:	Acetyl-CoA + histone = CoA + acetylhistone.
Enzyme Regulation:	Coactivator activity on nuclear receptors and NF-kappa-B pathways is enhanced by various hormones and the TNF cytokine respectively. TNF stimulation probably enhances phosphorylation which in turn activates coactivator function. In contrast acetylation by CREBBP apparently suppresses coactivation of target genes by disrupting its association with nuclear receptors.
Subunit:	Interacts with CARM1 (By similarity). Present in a complex containing NCOA2 IKKA IKKB IKBKG and the histone acetyltransferase protein CREBBP. Interacts with CASP8AP2 NR3C1 and PCAF. Interacts with ATAD2 and this interaction is enhanced by estradiol.
Subcellular Location:	Cytoplasm. Nucleus. Note=Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation it translocates from the cytoplasm to the nucleus.
Tissue Specificity:	Widely expressed. High expression in heart skeletal muscle pancreas and placenta. Low expression in brain and very low in lung liver and kidney.
Domain:	Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors and constitute the RID domain (Receptor-interacting domain).
Ptm:	Acetylated by CREBBP. Acetylation occurs in the RID domain and disrupts the interaction with nuclear receptors and regulates its function.
Ptm:	Methylated by CARM1 (By similarity).
Ptm:	Phosphorylated by IKK complex. Regulated its function.
Polymorphism:	The length of the poly-Gln region is polymorphic in the normal population.
Miscellaneous:	NCOA3 is frequently amplified or overexpressed in breast and ovarian cancers.
Similarity:	Belongs to the SRC/p160 nuclear receptor coactivator family.
Similarity:	Contains 1 basic helix-loop-helix (bHLH) domain.
Similarity:	Contains 1 PAS (PER-ARNT-SIM) domain.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.05 ml

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Delivery expected until 9/11/2025

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