PRMT5 [PRMT5-21]

GWB-3826D7

IgG2a

Human

PRMT5

2 mg/ml Storage

ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 2 µ g/ml (determined by using a whole cell extract of human melanoma cell line G361). Predicted

Nuclear Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins (Histone 2A 3 4) IL enhancer binding factor nuclear factors (NF45 90 ILF3 Nucleolin STAT1 Poly(A) binding protein II) cell cycle proteins (phosphoprotein phosphatase 2A) signal transduction proteins (FGF2 Fibrillarin FN INFAR1 Jak MBP Src-adaptor Sam68) apoptosis proteins (FADD ICE-like protease) and viral proteins (Hepatitis C NS3 RNA Helicase HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

S-adenosyl-L-methionine + histone-arginine = S-adenosyl-L-homocysteine + histone-N(omega)-methyl-arginine.

Cytoplasm.

Disulfide bonds and non-covalent association mediate homooligomers formation.