RPA34 [RPA34-20]

RPA34-20

Replication protein A purified from U293 cells.

HeLa or U293 cells or colon carcinoma tissue

Nuclear

From hybridoma culture supernatant Storage

For ICC: Use at an assay dependent dilution. For IF: Use at a concentration of 2-3 µ g/ml. For IHC-P: Use at a dilution of 2-3µ g/ml. For IP: Use at a dilution of 1µ g/reaction. For WB: Use at a dilution of 4-6µ g/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcjer. This antibody can be used as a marker for proliferation. Reacts with both the complexed (phosphorylated) form of the protein as well as the free (unphosphorylated) subunit. For paraffin sections pretreat with heat or use pressure cooker method. Target protein

Nuclear RPA is involved in DNA replication repair and recombination. Human RPA is a stable heterotrimer of 70 kDa 32-34 kDa and 11-14 kDa subunits (RPA70 RPA32 and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding for SV40 DNA and stimulates DNA polymerase alpha and delta.

Heterotrimer of 70 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.

Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA and by CDC2.