HNRNP M1-M4 [3C181]

3C181

M19 fusion protein containing full length native protein (purified) (Human).

Specific for human RNP M1-M4.

0. 75 mg/ml Storage

IP: Use at a dilution of 3/25. WB: Use at a dilution of 1/500. Detects a band at 64-68kD using HeLa cell lysate. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Proteins that directly bind to nascent RNA polymerase II transcripts the heterogenous nuclear ribonucleoproteins (hnRNPs) play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs the M1-M4 proteins appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64 kDa subunit of cleavage stimulation factor which is involved in 3-end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.

Identified in the spliceosome C complex at least composed of AQR ASCC3L1 C19orf29 CDC40 CDC5L CRNKL1 DDX23 DDX41 DDX48 DDX5 DGCR14 DHX35 DHX38 DHX8 EFTUD2 FRG1 GPATC1 HNRPA1 HNRPA2B1 HNRPA3 HNRPC HNRPF HNRPH1 HNRPK HNRNPM HNRPR HNRPU KIAA1160 KIAA1604 LSM2 LSM3 MAGOH MORG1 PABPC1 PLRG1 PNN PPIE PPIL1 PPIL3 PPWD1 PRPF19 PRPF4B PRPF6 PRPF8 RALY RBM22 RBM8A RBMX SART1 SF3A1 SF3A2 SF3A3 SF3B1 SF3B2 SF3B3 SFRS1 SKIV2L2 SNRPA1 SNRPB SNRPB2 SNRPD1 SNRPD2 SNRPD3 SNRPE SNRPF SNRPG SNW1 SRRM1 SRRM2 SYF2 SYNCRIP TFIP11 THOC4 U2AF1 WDR57 XAB2 and ZCCHC8.

Sumoylated.