Comparison

CD45 [MEM-28]

Item no. 20-272-192779
Manufacturer GENWAY
Amount 0.1 mg
Category
Type Antibody
Applications WB, IP, IHC
Clone MEM-28
Specific against other
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias GWB-14FA21
Similar products 20-272-192779
Available
Genway ID:
GWB-14FA21
Clone:
MEM-28
Isotype:
IgG1
Immunogen:
Tissue / cell preparation (Human). (Thymocytes and T lymphocytes).
Antigen Species:
Human
Specificity:
This product reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen) a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin except erythrocytes and platelets.
Positive Control:
Jurkat human leukemia T-cell lysate and Kg-1a human leukemia T-cell lysate
Target:
CD45
Localization:
Type I membrane protein
Concentration:
1 mg/ml
Purification Note:
From mouse ascitic fluid. Storage
Buffer:
Phosphate buffered saline pH 7. 4 containing 15mM sodium azide
Application Note:
For IHC-P: Use at an assay dependent dilution. No pre-treatment of tissue sections is essential. For WB: Use at a concentration of 2 µ g/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Cellular
Localization:
Type I membrane protein CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction inhibition or upregulation of various immunological functions.
Function:
Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation recruits an dephosphorylates SKAP1 and FYN.
Catalytic Activity:
Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit:
Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Domain:
The first PTPase domain interacts with SKAP1.
Ptm:
Heavily N- and O-glycosylated.
Disease:
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+)SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity leukopenia and low or absent antibody levels. Patients with SCID present in infancy with recurrent persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Disease:
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Similarity:
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Similarity:
Contains 2 fibronectin type-III domains.
Similarity:
Contains 2 tyrosine-protein phosphatase domains.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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