BRCA1 [17F8]

GWB-6B3E8D

IgG1Light chain: kappa

This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines BRCA1 aberrantly mislocates to the cytoplasm.

BRCA1

1 mg/ml

Phosphate-buffered saline pH7. 2 containing no preservatives

208 kDa. For IHC-P: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcherTarget protein

Nuclear/Cytoplasmic Expression system: pGEX-3X Myeloma: NS1 BRCA1-17F8 recognizes full-length BRCA1 a 220kDa nuclear phosphoprotein and does not recognize the ?exon 11 splice variant. In a high proportion of breast and ovarian cancer cell lines BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.

Part of the BRCA1-associated genome surveillance complex (BASC) which contains BRCA1 MSH2 MSH6 MLH1 ATM BLM PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts (via BRCT domains) with RBBP8. Associates with RNA polymerase II holoenzyme. Interacts with SMC1A and COBRA1/NELFB. Interacts (via BRCT domains) with BRIP1. Interacts with FANCD2 (ubiquitinated). Interacts with BAP1. Interacts with DCLRE1C/Artemis and CLSPN. Interacts with H2AFX (phosphorylated on \' Ser-140\' ). Interacts with CHEK1/CHK1. Interacts with BRCC3. Interacts (via BRCT domains) with ACACA (phosphorylated); the interaction prevents dephosphorylation of ACACA.

Isoform 3: Cytoplasm.

Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.

Phosphorylated in response to IR UV and various stimuli that cause checkpoint activation probably by ATM or ATR.

Defects in BRCA1 are a cause of genetic susceptibility to breast cancer (BC) [MIM:113705 114480]. BC is an extremely common malignancy affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease and this link is striking for early-onset breast cancer. Mutations in BRCA1 are thought to be responsible for 45% of inherited breast cancer. Moreover BRCA1 carriers have a 4-fold increased risk of colon cancer whereas male carriers face a 3-fold increased risk of prostate cancer. Cells lacking BRCA1 show defects in DNA repair by homologous recombination.

Defects in BRCA1 are a cause of genetic susceptibility to ovarian cancer [MIM:113705].

Contains 1 RING-type zinc finger.