SRC1 [1135]

GWB-923CF3

IgG1Light chain: kappa

Human

SRC1

From hybridoma tissue culture supernatant Storage

For IP: Use at a concentration of 0. 5 - 2 µ g/ml. For WB: Use at a concentration of 0. 5 - 2 µ g/ml. Predicted

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors such as for steroids (PGR GR and ER) retinoids (RXRs) thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3 STAT5A STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

Interacts with the methyltransferase CARM1 (By similarity). Interacts with NCOA6 and NCOA2. Interacts with the FDL motif of STAT5A and STAT5B. Interacts with the LXXLL motif of STAT6. Interacts with STAT3 following IL-6 stimulation. Interacts with the basal transcription factor GTF2B. Interacts with the histone acetyltransferases EP300 and CREBBP. Interacts with PCAF COPS5 NR3C1 and TTLL5/STAMP.

Widely expressed.

Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7 which is not present in isoform 2 increases the affinity for steroid receptors in vitro.

Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.

A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2; 2)(q35; p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood representing 5-8% of all malignancies in children.

Contains 1 basic helix-loop-helix (bHLH) domain.