PCNA [339]

GWB-C05239

IgG

Human

1 mg/ml

Phosphate-buffered saline pH 7. 2 containing no preservatives

31. 32 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Expression system: pGEX-3X Myeloma: NS1 PCNA is a homotrimeric protein that is involved in DNA replication and DNA repair. It serves as a processivity factor. for DNA polymerase d. It has also been shown to interact with several other proteins that are involved in DNA replication and repair including replication factor C DNA ligase I XPG FEN1 and p21. This partiular antiserum was raised against the interdomain connector loop region of PCNA which is the region of PCNA through which many of the protein-protein interactions occur.

Homotrimer. Interacts with KCTD10. Interacts with PPP1R15A (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH POLK DNMT1 ERCC5/XPG FEN1 CDC6 APEX2 and POLDIP2. Interacts with EXO1 and SHPRH. Forms a ternary complex with DNTTIP2 and core histone. Interacts with POLD1 POLD3 and POLD4.

Upon methyl methanesulfonate-induced DNA damage mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through \' Lys-63\' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligase SHPRH whih is required for DNA repair.

Belongs to the PCNA family.