CD4 [YTS 177.9] (FITC)

GWB-2AF9FC

IgG2a

Mouse

CD4

0. 1 mg/ml

Phosphate buffered saline pH7. 2 containing 1% BSA and 0. 09% sodium azide

Use 10ul of the suggested working dilution (Neat-1:10) to label 10^6 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Myeloma: Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1. 2. 3 rat myeloma cell line. CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (\" helper/inducer\" T cells) and is also expressed at a lower level on monocytes tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins.

Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.