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CD18 [MEM-48]

CD18 [MEM-48]

Item no.	20-272-194015
Manufacturer	GENWAY
Amount	0.1 mg
Category	Oncology Antibodies Immunoprecipitation Primary Antibodies By Organ Heart & Blood Cancer
Type	Antibody
Applications	IP
Clone	MEM-48
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-BC9749
Similar products	20-272-194015
Available
Genway ID:	GWB-BC9749
Clone:	MEM-48
Isotype:	IgG1
Immunogen:	Leucocytes of a patient suffering from a LGL-type leukaemia.
Specificity:	The antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (Integrin beta2 chain).
Target:	CD18
Concentration:	1 mg/ml
Purification Note:	Purified from ascites by protein-A affinity chromatography. Storage
Buffer:	Azide free phosphate buffered saline (PBS) preserved by filter sterilization approx. pH 7. 4
Application Note:	Flow Cytometry; Immunoprecipitation;
Western Blotting:	Recommended
Dilution:	2 _g/ml
Positive Control:	Kg-1a human leukemia cell lysate Negative control:JURKAT human leukemia T-cell lysate Sample
Preparation:	buffer with laurylmaltoside 2 x non-reducing SDS
Application Note:	Non-reducing conditions. SDS-PAGE (6% separating gel 4% stacking gel). Recommended secondary antibody - anti mouse IgG/HRP dilution 1:2000 60 min on vertical incubator. Immunohistochemistry (frozen section); Functional Application: The antibody induces high-affinity conformation of LFA-1 complex.
Function:	Integrin alpha-L/beta-2 is a receptor for ICAM1 ICAM2 ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Subunit:	Heterodimer of an alpha and a beta subunit. Beta-2 associates with either alpha-L alpha-M alpha-X or alpha-D. Interacts with COPS5 and RANBP9.
Subcellular Location:	Membrane; Single-pass type I membrane protein.
Ptm:	Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
Disease:	Defects in ITGB2 are the cause of leukocyte adhesion deficiency type I (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Similarity:	Belongs to the integrin beta chain family.
Similarity:	Contains 1 VWFA domain.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 9/11/2025

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