MYELOPEROXIDASE (MPO)

GWB-C0E987

The monoclonal antibody 8F4 can be used for immuno assays. flow cytometry and immunohistology on frozen sections. The monoclonal antibody 8F4 reacts with mouse myeloperoxidase (MPO). MPO is a glycoprotein with an alpha2beta2 heteromultimer expressed in all cells of the myeloid linage. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils. It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl). a potent oxidant. MPO is rapidly released by activated polymorphonuclear neutrophils. Involvement of MPO has been described in numerous diseases such as atherosclerosis. lung cancer. Alzheimer\' s disease and multiple sclerosis. Autoimmune antibodies to MPO are involved in Wegeners disease. Since the discovery of MPO deficiency. initially regarded as rare and restricted to patients suffering from severe infections. MPO has more attracted clinical attention. In experimental studies antibodies to MPO can be used for various purposes ranging from flow cytometric analysis to detection of polymorphonuclear neutrophils in tissue sections. Monoclonal antibody 8F4 also reacts with rat MPO.

Donor + H2O2 = oxidized donor + 2 H2O.

Tetramer of two light chains and two heavy chains.

Belongs to the peroxidase family. XPO subfamily. [1] Ostanin D. V. Barlow S. Shukla D. and Grisham M. B. et al. NADPH oxidase but not myeloperoxidase protects lymphopenic mice from spontaneous infections[2] Li P. Ogino K. Hoshikawa Y. Morisaki H. Cheng J. Toyama K. Morisaki T. Hashimoto K. Ninomiya H. Tomikura-Shimoyama Y. et al. Remote reperfusion lung injury is associated with AMP deaminase 3 activation and attenuated by inosine monophosphate[3] Xiao H. Schreiber A. Heeringa P. Falk R. J. and Jennette J. C. et al. Alternative complement pathway in the pathogenesis of disease mediated by anti-neutrophil cytoplasmic autoantibodies[4] Reynolds W. F. Kumar A. P. and Piedrafita F. J. et al. The human myeloperoxidase gene is regulated by LXR and PPARalpha ligands[5] Borrego A. Peters L. C. Jensen J. R. Ribeiro O. G. Koury Cabrera W. H. Starobinas N. Seman M. Ibanez O. M. and De Franco M. Genetic determinants of acute inflammation regulate Salmonella infection and modulate Slc11a1 gene (formerly Nramp1) effects in selected mouse lines[6] Venturelli D. Shirsat N. Gemperlein I. Bittenbender S. Rovera G. Nucleotide sequence of cDNA for murine myeloperoxidase. [7] Venturelli D. Bittenbender S. Rovera G. Sequence of the murine myeloperoxidase (MPO) gene.