Alpha-I Spectrin (erythroid)

GWB-6654AB

17C7Host Animal: Mouse

This antibody was developed against the alpha I spectrin SH3 domain. (2) Specific marker for human. rat and mouse alpha I spectrin. Reacts with erythroid spectrin in red blood cells and in cerebellum. Does not cross react with alpha II (non-erythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In cerebellar cell cultures. clone 17C7 labels cell bodies consistent with localization of erythroid spectrin. In NIH 3T3 cells. clone 17C7 reacts with a 200kDa protein which is a candidate marker of endocytic vesicles called macropinosomes. (1. 2. 5)

1mg/mlPreservatives: NaN3

This product contains sodium azide which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0. 1â ??1. 0%. When disposing of this reagent through lead or copper plumbing flush with copious volumes of water to prevent azide build-up in drains. MAb to Alpha-I Spectrin (erythroid). Monoclonal Antibody to Human Spectrin alpha-I SH3 domain (erythroid)

Composed of nonhomologous chains alpha and beta which aggregate to form dimers tetramers and higher polymers.

This complex is anchored to the cytoplasmic face of the plasma membrane via another protein ankyrin which binds to beta-spectrin and mediates the binding of the whole complex to a transmembrane protein band 3. The interaction of erythrocyte spectrin with other proteins through specific binding domains lead to the formation of an extensive subplasmalemmal meshwork which is thought to be responsible for the maintenance of the biconcave shape of human erythrocytes for the regulation of plasma membrane components and for the maintenance of the lipid asymmetry of the plasma membrane.

Contains 3 EF-hand domains.

Contains 22 spectrin repeats.