Low Density LRP (85kDa)

GWB-DAEB51

8B8Host Animal: Mouse

Monoclonal Antibodies to Lipoproteins

0. 05M Sodium phosphate pH 6. 6 containing 0. 1M Sodium chloride and 1mM EDTAApplications Notes : Western blot (non-reducing conditions): 1?5ug/ml. Immunohistochemistry using paraffin and frozen sections: 1?10ug/ml. Flow cytometry will recognize an epitope on the extracellular domain. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. MAb to Low Density LRP (85kDa). Monoclonal Antibody to Human Low Density Lipoprotein Receptor-Related Protein (LRP) 85kDa

Heterodimer of an 85-kDa membrane-bound carboxyl subunit and a non-covalently attached 515-kDa amino-terminal subunit. Intracellular domain interacts with MAFB (By similarity). Found in a complex with PID1/PCLI1 LRP1 and CUBNI. Interacts with SNX17 PID1/PCLI1 PDGF and CUBN. The intracellular domain interacts with SHC1 GULP1 and DAB1. Interacts with LRPAP1.

Cell membrane; Single-pass type I membrane protein. Membrane coated pit.

Cell membrane; Peripheral membrane protein; Extracellular side. Membrane coated pit.

Cytoplasm. Nucleus. Note=After cleavage the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus.

Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.

Phosphorylated on serine and threonine residues.

Belongs to the LDLR family.

Contains 31 LDL-receptor class A domains.