Actin (alpha smooth muscle)

GWB-A0AB1B

a-SM1

Each vial contains 100 ?l 1 mg/ml purified antibody in PBS containing 0. 09% sodium azide. Among the six actin isoforms described in mammals. two are found in virtually all cells (?? and ??cytoplasmic). two are detected in smooth muscle cells (?? and ?-smooth muscle) and two are present in striated muscles. one predominantly in skeletal (?-skeletal) and one in cardiac (?-cardiac) muscle cells. These actin isoforms differ slightly in their Nterminus and the sequences of each of them are perfectly conserved in higher vertebrates. Alphasmooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition. it has also been shown to appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases

Homodimer; antiparallel. Interacts with MYOZ2 PDLIM2 TTID and LPP (By similarity).

Belongs to the alpha-actinin family.

Contains 2 CH (calponin-homology) domains.

Contains 4 spectrin repeats. Skalli. O. Ropraz. P. . Trzeciak. A. . Benzonana. G. . Gillessen. D. . and Gabbiani. G. (1986). A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 103. 2787-2796. 2. Skalli. O. . Schurch. W. . Seemayer. T. . Lagace. R. . Montandon. D. . Pittet. B. . and Gabbiani. G. (1989). Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins. Lab Invest 60. 275-285. 3. Sappino. A. P. Schurch. W. . and Gabbiani. G. (1990). Differentiation repertoire of fibroblastic cells: expression of cytoskeletal proteins as marker of phenotypic modulations. Lab Invest 63. 144-161. 4. Vyalov. S. L. . Gabbiani. G. . and Kapanci. Y. (1993). Rat alveolar myofibroblasts acquire alpha-smooth muscle actin expression during bleomycin-induced pulmonary fibrosis. Am J Pathol 143. 1754-1765. 5. Chaponnier. C. . Goethals. M. . Janmey. P. A. . Gabbiani. F. . Gabbiani. G. . and Vandekerckhove. J. (1995). The specific NH2- terminal sequence Ac-EEED of alpha-smooth muscle actin plays a role in polymerization in vitro and in vivo. J Cell Biol 130. 887-895. 6. Hinz. B. . Celetta. G. . Tomasek. J. J. . Gabbiani. G. . and Chaponnier. C. (2001). Alpha-smooth muscle actin expression upregulates fibroblast contractile activity. Mol Biol Cell 12. 2730-2741. 7. Hinz. B. . Gabbiani. G. and Chaponnier. C. (2002). The NH2-terminal peptide of alphasmooth muscle actin inhibits force generation by the myofibroblast in vitro and in vivo. J Cell Biol 157. 657-663.