Antibodies

CD71

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against other
Clone YTA74. 4
Applications FC, IP, IHC
Amount 0.025 mg
ArtNr 20-783-310014
Available
Genway ID:
GWB-82F042
Specificity:
CD71NCBI
Gene ID:
22042
Specificity:
CD71
Clone:
YTA74. 4
Immunogen:
Mouse Concanavilin A activated spleen cells.
Fusion Partner:
Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1. 2. 3 rat myeloma cell line.
Specificity Note:
Mouse CD71 antigen the transferrin receptor (TFR). Expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90kD by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217. 1. 3. and R17 208. 2 anti-TFR monoclonal antibodies (1 2).
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers:
0. 09%Sodium AzideSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. Suggested
Dilution:
Flow Cytometry - 1/50 - 1/100
Function:
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand the heditary hemochromatosis protein HFE competes for binding with transferrin for an overlapping C-terminal binding site.
Subunit:
Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen DR1. Interacts with SH3BP3.
Subcellular Location:
Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Subcellular Location:
Transferrin receptor protein 1 serum form: Secreted.
Induction:
Regulated by cellular iron levels through binding of the iron regulatory proteins IRP1 and IRP2 to iron-responsive elements in the 3\' -UTR. Up-regulated upon mitogenic stimulation.
Ptm:
N- and O-glycosylated phosphorylated and palmitoylated. The serum form is only glycosylated.
Ptm:
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Ptm:
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Miscellaneous:
Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemia resulting from a number of conditions including rheumatoid arthritis pregnancy irritable bowel syndrome and in HIV patients.
Miscellaneous:
Canine and feline parvoviruses bind human and feline transferrin receptors and use these receptors to enter and infect cells.
Similarity:
Belongs to the peptidase M28 family. M28B subfamily [view classification].
Similarity:
Contains 1 PA (protease associated) domain. 1. Lesley. J. et al. (1984) Expression of transferrin receptor on murine haematopoietic progenitors. 2. Trowbridge. I. S. et al. (1982) Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody.
Amount: 0.025 mg
Available: In stock
Listprice: €152.38
Price: €152.38
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