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CD161b/c

CD161b/c


Manufacturer	GENWAY
Category	Oncology Antibodies Flow Cytometry Primary Antibodies By Organ Bone Cancer
Type	Antibody
Specific against	other
Clone	PK136
Applications	FC
Amount	0.025 mg
Item no.	20-783-310042
eClass 6.1	32160702
eClass 9.0	32160702
Available
Genway ID:	GWB-E9106C
Specificity:	CD161b/cNCBI
Gene ID:	17059
Specificity:	CD161b/c
Clone:	PK136
Immunogen:	Spleen and bone marrow cells from CE mice
Fusion Partner:	Spleen cells from immunised (C3H x BALB/c) FI Hybrid were fused with cells of the Sp2/0 - Ag14 myeloma cell line.
Preparation:	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution:	Phosphate buffered saline pH7. 4
Preservative Stabilisers:		0. 09%Sodium Azide1%Bovine Serum AlbuminSuggested
Flow Cytometry:	Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (/). Suggested
Dilution:	Flow Cytometry - Neat
Function:	Plays a stimulatory role on natural killer (NK) cells cytotoxicity. Activation by cross-linking of the receptor induces Ca(2+) mobilization and interferon-gamma production.
Subunit:	Homodimer; disulfide-linked. Interacts with tyrosine kinase LCK.
Subcellular Location:	Membrane; Single-pass type II membrane protein.
Tissue Specificity:	Expressed in natural killer cells.
Miscellaneous:	The anti-NK1. 1 monoclonal antibody (PK136) recognizing Klrb1b and Klrb1c genes is known to identify NK cells from B6 and SLJ mice but not from BALB/c ones. A single mutation Thr-191 present in Klrb1b and Klrb1c genes of BALB/c mice may be responsible for lack of NK1. 1 reactivity in these mice. Indeed when Lys-217 of BALB/c mice Klrb1c is mutated to Glu-217 present in B6 and SLJ mice it does not confer NK1. 1 reactivity.
Similarity:	Contains 1 C-type lectin domain. 1. Koo. G. C. and Peppard. J. R. (1981) Establishment of monoclonal anti-NK-1. 1 antibody. 2. Koo. G. C. et al. (1986) The NK-1. 1 (-) mouse: A model to study differentiation of murine NK cells. 3. Kung. S. K. P. and Miller. R. G. (1995) The NK1. 1 antigen in NK-mediated F1 antiparent killing in vitro. 4. Wang. M. et al. (1998) Natural killer cell depletion fails to influence initial CD4 T cell commitment in vivo in exogenous antigen stimulated cytokine and antibody responses. 5. Arase. N. et al. (1997) Association with FcRgamma is essential for activation signal through NKR-P1 (CD161) in natural killer (NK) cells and NK1. 1+ T cells. 6. Kung. S. K. P. et al. (1999) The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells. 7. Carlyle. J. R. et al. (1999) Mouse NKR-P1B. a novel NK1. 1 antigen with inhibitory function.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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Amount: 0.025 mg

available

Delivery expected until 5/9/2024