CD247

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Clone G3
Applications FC
Amount 25 TESTS
ArtNr 20-783-310049
Available
Genway ID:
GWB-70F117
Specificity:
CD247NCBI
Gene ID:
919
Isotype:
IgG2a
Clone:
G3
Product Form:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Immunogen:
113 amino acid synthetic peptide corresponding to the cytoplasmic domain of murine zeta TCR subunit.
Fusion Partner:
Spleen cells from immunised BALB/c mice were fused with cells of the Ag8. 653 mouse myeloma cell line.
Specificity Note:
The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events. A novel monoclonal antibody named G3 has been developed that is specific for the TCR zeta chain subunit (CD247). The antibody is capable of precipitating both non-phosphorylated and tyrosine phosphorylated TCR zeta chain and may be used to detect TCR zeta expressing cells using flow cytometry on permeabilised cells.
Preparation:
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 2
Preservative Stabilisers:
0. 09% Sodium Azide 1% Bovine Serum Albumin
Reconstitution: Reconstitute in 0. 25 ml disilled waterSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood. Membrane permeabilisation is required for this application using a digitonin based method. Suggested
Dilution:
Flow Cytometry - Neat
Function:
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Subunit:
The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma delta epsilon zeta and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Interacts with DOCK2 and TRAT1. Interacts with HIV-1 Nef protein. Interacts with SHB.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Domain:
The ITAM domains mediate interaction with SHB.
Ptm:
Phosphorylated on Tyr residues after T-cell receptor triggering (By similarity).
Disease:
Defects in CD247 are a cause of primary T-cell immunodeficiency [MIM:610163]. Affected individuals suffer of recurrent infections. Patients T-cell counts are very low and B-cell counts are normal.
Similarity:
Belongs to the CD3Z/FCER1G family.
Similarity:
Contains 3 ITAM domains. 1. van Oers. N. S. C. et al. (1994) Production and characterisation of monoclonal antibodies specific for the murine T cell receptor zeta chain. 2. Anderson. P. (1995) mAb reative with lymphocyte restricted intracellular antigens. Leucocyte Typing V p325. Oxford University Press.
Amount: 25 TESTS
Available: In stock
Listprice: €258.32
Discount: -10.0%
Price: €232.49
You save: €25.83
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