CD247

GWB-70F117

919

G3

113 amino acid synthetic peptide corresponding to the cytoplasmic domain of murine zeta TCR subunit.

The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events. A novel monoclonal antibody named G3 has been developed that is specific for the TCR zeta chain subunit (CD247). The antibody is capable of precipitating both non-phosphorylated and tyrosine phosphorylated TCR zeta chain and may be used to detect TCR zeta expressing cells using flow cytometry on permeabilised cells.

Phosphate buffered saline pH7. 2

Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood. Membrane permeabilisation is required for this application using a digitonin based method. Suggested

Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

Membrane; Single-pass type I membrane protein.

Phosphorylated on Tyr residues after T-cell receptor triggering (By similarity).

Belongs to the CD3Z/FCER1G family.