CD59

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Clone MEM-43/5
Applications FC
Amount 25 TESTS
ArtNr 20-783-310192
Available
Genway ID:
GWB-E738D1
Specificity:
CD59NCBI
Gene ID:
966
Specificity:
CD59
Clone:
MEM-43/5
Preparation:
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution:
Phosphate buffered saline
Preservative Stabilisers:
0. 09%Sodium Azide1%Bovine Serum Albumin
Reconstitution: Reconstitute in 0. 25 ml disilled waterSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:
Flow Cytometry - Neat
Function:
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
Function:
The soluble form from urine retains its specific complement binding activity but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
Subunit:
Interacts with T-cell surface antigen CD2.
Subcellular Location:
Cell membrane; Lipid-anchor GPI-anchor. Secreted. Note=Soluble form found in a number of tissues.
Ptm:
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide Gal-beta-1 3GalNAc and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Ptm:
Glycated. Glycation is found in diabetic subjects but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
Similarity:
Contains 1 UPAR/Ly6 domain. 1. Stefanova. I. et al. (1989) Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol Mol. Immunol. 26: 153-161. 2. Stefanova. I. . et al. (1989) in Leucocyte Typing IV: White cell differentiation antigens. Ed. Knapp. W. et al. Oxford University Press pp 678-697. 3. Tandon. N. et al. (1994) Expression and function of multiple regulators of complement activation in autoimmune thyroid disease. 4. Horejsi. V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200). CD3 (T3). CD43. CD10 (CALLA). transferrin receptor (T9). a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). 5. Horejsi. V. et al. (1988) Title Fol. Bio. (Prague) 34: 23-24.
Amount: 25 TESTS
Available: In stock
Listprice: €212.88
Discount: -10.0%
Price: €191.59
You save: €21.29
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