CD195


Manufacturer	GENWAY
Category	Oncology Antibodies By Cell Type Carcinoma Flow Cytometry Primary Antibodies By Organ Lung Cancer
Type	Antibody
Specific against	other
Clone	HEK/1/85a
Applications	FC
Amount	0.025 mg
Item no.	20-783-310263
eClass 6.1	32160702
eClass 9.0	32160702
Available
Genway ID:	GWB-8415BD
Specificity:	CD195
Specificity:	CD195
Clone:	HEK/1/85a
Immunogen:	CHO cells transfected with human CCR5.
Fusion Partner:	Spleen cells from immunised rats were fused with cell of the rat Y3 myeloma cell line.
Preparation:	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:	Phosphate buffered saline
Preservative Stabilisers:		0. 09%Sodium AzideSuggested
Flow Cytometry:	Use 10ul of the suggested working dilution to label 100ul fresh whole blood. Suggested
Dilution:	Flow Cytometry - 1/50 - 1/100
Function:	Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
Subunit:	Interacts with PRAF2. Interacts with HIV-1 surface protein gp120. Efficient ligand binding to CCL3/MIP-1alpha and CCR4/MIP-1beta requires sulfation O-glycosylation and sialic acid modifications. Glycosylation on Ser-6 is required for efficient binding of CCL4. Interacts with ADRBK1.
Subcellular Location:	Cell membrane; Multi-pass membrane protein.
Tissue Specificity:	Highly expressed in spleen thymus in the myeloid cell line THP-1 in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
Ptm:	Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines CCL3 and CCL4.
Ptm:	O-glycosylated but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and if so with small moieties such as a T-antigen.
Ptm:	Palmitoylation in the C-terminal is important for cell surface expression and to a lesser extent for HIV entry.
Ptm:	Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
Polymorphism:	Variations in CCR5 are associated with resistance or susceptibility to immunodeficiency virus type 1 (resistance or susceptibility to HIV-1) [MIM:609423]. Variations in CCR5 gene also influence the rate of progression to AIDS after infection.
Polymorphism:	Ser-60 variant a naturally occurring mutation in a conserved residue in the first intracellular domain of CCR5 results in reduced amounts of the protein in the membrane and consequently may be associated with reduced susceptibility to infection by microbes that depend on these molecules as their receptors.
Polymorphism:	Variations in CCR5 are associated with susceptibility to West Nile virus (WNV) infection [MIM:610379].
Similarity:	Belongs to the G-protein coupled receptor 1 family [view classification]. 1. Mueller. A. et al. (2002) Pathways for internalization and recycling of the chemokine receptor CCR5.

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Amount: 0.025 mg

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Delivery expected until 5/30/2024