CD162


Manufacturer	GENWAY
Category	Antibodies Cell Biology Cell Movement Cell Adhesion Flow Cytometry Immunoprecipitation Primary Antibodies
Type	Antibody
Specific against	other
Clone	TB5
Applications	FC, IP
Amount	0.025 mg
Item no.	20-783-310291
eClass 6.1	32160702
eClass 9.0	32160702
Available
Genway ID:	GWB-59BCB0
Specificity:	CD162NCBI
Gene ID:	6404
Specificity:	CD162
Clone:	TB5
Preparation:	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:	Phosphate buffered saline pH7. 4
Preservative Stabilisers:		0. 09%Sodium AzideSuggested
Flow Cytometry:	Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:	Flow Cytometry - 1/10
Function:	A SLe(x)-type glycan which through high affinity calcium-dependent interactions with E- P- and L-selectins mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.
Subunit:	Homodimer; disulfide-linked. Interaction with P- E- and L-selectins through their lectin/EGF domains is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Sulfation on Tyr-51 of PSGL1 is most important for high affinity L-selectin/SELL binding while P-selectin/SELP requires sulfation on Tyr-48. E-selectin/SELE binds with much lower affinity and requires the sLe(x) epitope but apparantly not tyrosine sulfation. Dimerization appears not to be required for P-selectin/SELP binding. Interacts with SNX20.
Subcellular Location:	Membrane; Single-pass type I membrane protein.
Tissue Specificity:	Expressed on neutrophils monocytes and most lymphocytes.
Ptm:	Displays complex core-2 sialylated and fucosylated O-linked oligosaccharides at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide Galbeta1--& gt; 4GlcNAcbeta1--& gt; 6(Galbeta1--& gt; 3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1 3 linkage present in two forms: One species is a disialylated monofucosylated glycan and the other a monosialylated trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Ptm:	Sulfation in conjunction with the SLe(x)-containing glycan is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51 whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment and leukocyte rolling.

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Amount: 0.025 mg

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Delivery expected until 5/9/2024