CD209

CD209

MR-1

Spleen cells from immunised Balb/c mice were fused with cells of the Mouse SP2/0-Ag14 myeloma cell line.

Phosphate buffered saline pH7. 4

Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested

Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens including HIV-1 gp120 HIV-2 gp120 SIV gp120 ebolavirus glycoproteins cytomegalovirus gB HCV E2 dengue virus gE Leishmania pifanoi LPG Lewis-x antigen in Helicobacter pylori LPS mannose in Klebsiella pneumonae LPS di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.

Homotetramer. Binds to many viral surface glycoproteins such as HIV-1 gp120 HIV-2 gp120 SIV gp120 ebolavirus envelope glycoproteins cytomegalovirus gB HCV E2 and dengue virus major envelope protein E.

Isoform 2: Cell membrane; Single-pass type II membrane protein (Probable).

Isoform 4: Cell membrane; Single-pass type II membrane protein (Probable).

Isoform 6: Secreted (Probable).

Isoform 8: Secreted (Probable).

Isoform 10: Secreted (Probable).

Isoform 12: Secreted (Probable).

The tandem repeat domain also called neck domain mediates oligermerization.

In vitro is a receptor for HIV-1 and transmits HIV-1 either in trans without DC infection or in cis following a DC infection to permissive T-cells to induce a robust infection. Bound HIV-1 remains infectious over a prolonged period of time and it is proposed that bound HIV-1 is not degraded but protected in non-lysosomal acidic organelles within the DCs close to the cell membrane thus contributing to the HIV-1 infectious potential during transport by DCs from the periphery to lymphoid organs.