CD209

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Clone MR-1
Applications FC
Amount 25 TESTS
ArtNr 20-783-310372
Available
Genway ID:
GWB-6413D5
Specificity:
CD209
Specificity:
CD209
Clone:
MR-1
Immunogen:
Immature myeloid monocyte-derived dendritic cells (MDDCs)
Fusion Partner:
Spleen cells from immunised Balb/c mice were fused with cells of the Mouse SP2/0-Ag14 myeloma cell line.
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09%Sodium Azide1%Bovine Serum Albumin
Reconstitution: Reconstitute in 0. 25 ml disilled waterSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:
Flow Cytometry - Neat - 1/10
Function:
Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens including HIV-1 gp120 HIV-2 gp120 SIV gp120 ebolavirus glycoproteins cytomegalovirus gB HCV E2 dengue virus gE Leishmania pifanoi LPG Lewis-x antigen in Helicobacter pylori LPS mannose in Klebsiella pneumonae LPS di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
Function:
On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
Subunit:
Homotetramer. Binds to many viral surface glycoproteins such as HIV-1 gp120 HIV-2 gp120 SIV gp120 ebolavirus envelope glycoproteins cytomegalovirus gB HCV E2 and dengue virus major envelope protein E.
Subcellular Location:
Isoform 1: Cell membrane; Single-pass type II membrane protein (Probable).
Subcellular Location:
Isoform 2: Cell membrane; Single-pass type II membrane protein (Probable).
Subcellular Location:
Isoform 3: Cell membrane; Single-pass type II membrane protein (Probable).
Subcellular Location:
Isoform 4: Cell membrane; Single-pass type II membrane protein (Probable).
Subcellular Location:
Isoform 5: Cell membrane; Single-pass type II membrane protein (Probable).
Subcellular Location:
Isoform 6: Secreted (Probable).
Subcellular Location:
Isoform 7: Secreted (Probable).
Subcellular Location:
Isoform 8: Secreted (Probable).
Subcellular Location:
Isoform 9: Secreted (Probable).
Subcellular Location:
Isoform 10: Secreted (Probable).
Subcellular Location:
Isoform 11: Secreted (Probable).
Subcellular Location:
Isoform 12: Secreted (Probable).
Tissue Specificity:
Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages endothelial cells of placental vascular channels peripheral blood mononuclear cells and THP-1 monocytes.
Domain:
The tandem repeat domain also called neck domain mediates oligermerization.
Polymorphism:
Genetic variations in CD209 determine Mycobacterium tuberculosis susceptibility [MIM:607948].
Miscellaneous:
In vitro is a receptor for HIV-1 and transmits HIV-1 either in trans without DC infection or in cis following a DC infection to permissive T-cells to induce a robust infection. Bound HIV-1 remains infectious over a prolonged period of time and it is proposed that bound HIV-1 is not degraded but protected in non-lysosomal acidic organelles within the DCs close to the cell membrane thus contributing to the HIV-1 infectious potential during transport by DCs from the periphery to lymphoid organs.
Similarity:
Contains 1 C-type lectin domain. 1. Relloso. M. et al. (2002) DC-SIGN (CD209) expression is IL-4 dependent and is negatively regulated by IFN. TGF-beta. and anti-inflammatory agents. 2. Melero. I. et al. (2002) An anti-ICAM-2 (CD102) monoclonal antibody induces immune-mediated regressions of transplanted ICAM-2-negative colon carcinomas.
Amount: 25 TESTS
Available: In stock
Listprice: €212.88
Discount: -10.0%
Price: €191.59
You save: €21.29
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