Manufacturer |
GENWAY
|
Category |
|
Type |
Antibody |
Specific against |
other |
Clone |
QBEND/10 |
Applications |
WB, FC, IP, IHC |
Amount |
0.025 mg |
Item no. |
20-783-310692 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Genway ID: |
GWB-876746 |
Specificity: |
CD34 CLASS II NCBI |
Gene ID: |
947 |
Specificity: |
CD34 CLASS II |
Clone: |
QBEND/10 |
Immunogen: |
Human endothelial cell membrane vesicles |
Fusion Partner: |
Spleen cells from immunised NZB mice were fused with cells of the mouse NSO myeloma cell line. |
Immunohistology: |
This product does not require antigen retreival using heat treatment or protein digestion. However staining of paraffin embedded formalin fixed tissues may be enhanced by enzyme pre-treatment using pronase or heat treatment using citrate buffer. Histology: Tonsil Bone Marrow. |
Preparation: |
Purified IgG prepared by affinity chromatography on Protein A |
Buffer Solution: |
Phosphate buffered saline |
|
Preservative Stabilisers: |
| 0. 09%Sodium AzideSuggested |
Flow Cytometry: |
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested |
Dilution: |
Flow Cytometry - 1/100 - 1/200Immunohistology - Paraffin - 1/500 - 1/1000 |
Function: |
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. |
Subcellular Location: |
Membrane; Single-pass type I membrane protein. |
Tissue Specificity: |
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Developmental Stage: On early hematopoietic progenitor cells. |
Ptm: |
Highly glycosylated. |
Ptm: |
Phosphorylated on serine residues by PKC. |
Disease: |
Abnormal CD34 expression in leukemogenesis. |
Similarity: |
Belongs to the CD34 family. 1. Fina. L. et al. (1990) Expression of the CD34 gene in vascular endothelial cells. 2. Sopper. S. et al. (1997) Lymphocyte subsets and expression of different markers in blood and lymphoid organs of rhesus monkeys. 3. Yoshino. N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts. cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. 4. Pammer. J. et al. (1996) CD40 antigen is expressed by endothelial cells and tumor cells in Kaposi\' s sarcoma. |
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