Antibodies

CD61

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against other
Clone PM6/13
Applications FC
Amount 0.025 mg
ArtNr 20-783-310710
Available
Genway ID:
GWB-7658D6
Specificity:
CD61NCBI
Gene ID:
3690
Specificity:
CD61
Clone:
PM6/13
Immunogen:
Platelet plasma membrane
Fusion Partner:
Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.
Preparation:
Purified IgG prepared by ion exchange chromatography
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09%Sodium Azide1%Bovine Serum AlbuminSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole bloodSuggested
Dilution:
Flow Cytometry - Neat
Function:
Integrin alpha-V/beta-3 is a receptor for cytotactin fibronectin laminin matrix metalloproteinase-2 osteopontin osteomodulin prothrombin thrombospondin vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin fibrinogen plasminogen prothrombin thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi\' s sarcoma lesions.
Subunit:
Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with HIV-1 Tat.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
Ptm:
Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. SHC binding also occurs when Tyr-785 alone is phosphorylated.
Polymorphism:
Position 59 is associated with platelet-specific alloantigen HPA-1 (ZW or PL(A)). HPA-1A/ZW(A)/PL(A1) has Leu-59 and HPA-1B/ZW(B)/PL(A2) has Pro-59.
Polymorphism:
Position 169 is associated with platelet-specific alloantigen HPA-4 (PEN or YUK). HPA-4A/PEN(A)/YUK(A) has Arg-169 and HPA-4B/PEN(B)/YUK(B) has Gln-169. HPA-4B is involved in neonatal alloimmune thrombocytopenia (NAIT or NATP).
Polymorphism:
Position 433 is associated with platelet-specific alloantigen MO. MO(-) has Pro-433 and MO(+) has Ala-433. MO(+) is involved in NAIT.
Polymorphism:
Position 515 is associated with platelet-specific alloantigen CA/TU. CA(-)/TU(-) has Arg-515 and CA(+)/TU(+) has Gln-515. CA(+) is involved in NAIT.
Polymorphism:
Position 662 is associated with platelet-specific alloantigen SR(A). SR(A)(-) has Arg-662 and SR(A)(+) has Cys-662.
Disease:
Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. Its inheritance is autosomal recessive. It is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I platelets show absence of the glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II the platelets express the GPIIb-IIIa complex at reduced levels (5-20% controls) have detectable amounts of fibrinogen and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors.
Similarity:
Belongs to the integrin beta chain family.
Similarity:
Contains 1 VWFA domain. 1. Michelson. A. D. et al. (1995) A panel of platelet mAb for the study of haemostasis and thrombosis in baboons. Leucocyte Typing V. Oxford University Press p 1230-1231. 2. Phillips. D. R. et al. (1991) GPIIb-IIIa: the responsive integrin. 3. Hynes. R. O. (1992) Integrins: versatility. modulation. and signaling in cell adhesion.
Amount: 0.025 mg
Available: In stock
Listprice: €212.88
Price: €212.88
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