Antibodies

MOUSE ANTI AMPK ALPHA 1/2

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against other
Applications WB, IF, IP
Amount 0.1 mg
Host Mouse
ArtNr 20-783-314673
Available
Genway ID:
GWB-E5FB3C
Specificity:
AMPK ALPHA 1/2
Isotype:
IgG2aSpecies Cross Reactivity: Based on sequence similarity is expected to react with:Vertebrates Human Mouse RatN. B. Antibody reactivity and working conditions may vary between species.
Preparation:
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09% Sodium Azide (NaN3)Approx. Protein Concentrations: IgG concentration 1. 0mg/ml
Immunogen:
29 amino acid peptide within the N-terminal catalytic domain of Drosophila AMPK alpha.
Specificity:
Recognises both the alpha 1 and the alpha 2 isoforms of AMP-activated protein kinase (AMPK). AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit which is stabilised by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia ischemia exercise low glucose levels. AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. Recent studies suggest that AMPK functions as a suppressor of cell proliferation of both malignant and normal cells. Recommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGHuCAL Anti Mouse IgG2aRabbit Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMGoat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L)
Function:
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5\' -AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Ref. 8Ref. 9Catalytic activityATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Enzyme regulationBinding of AMP results in allosteric activation inducing phosphorylation on Thr-183 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions without detectable changes in the AMP/ATP ratio. Ref. 8Ref. 9Subunit structureHeterotrimer of an alpha catalytic subunit a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2. Ref. 14Ref. 15Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain. Sequence cautionThe sequence AAA64850. 1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD43027. 1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAH37303. 1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAA36547. 1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG35788. 1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. [1] \" Isolation mapping and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. \" Matsushima M. Fujiwara T. Takahashi E. Minaguchi T. Eguchi Y. Tsujimoto Y. Suzumori K. Nakamura Y. Genes Chromosomes Cancer 21:230-235(1998) [PubMed: 9523198] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [2] \" BNIP3a a human homolog of pro-apoptotic protein BNIP3 promotes apoptosis and interacts with viral and cellular anti-apoptosis proteins. \" Yasuda M. Han J. -W. Dionne C. A. Boyd J. M. Chinnadurai G. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA]. [3] \" Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. \" Chen G. Cizeau J. Vande Velde C. Park J. H. Bozek G. Bolton J. Shi L. Dubik D. Greenberg A. J. Biol. Chem. 274:7-10(1999) [PubMed: 9867803] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal liver. [4] \" A novel adenovirus E1B19K-binding protein B5 inhibits apoptosis induced by Nip3 by forming a heterodimer through the C-terminal hydrophobic region. \" Ohi N. Tokunaga A. Tsunoda H. Nakano K. Haraguchi K. Oda K. Motoyama N. Nakajima T. Cell Death Differ. 6:314-325(1999) [PubMed: 10381623] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] FUNCTION SUBCELLULAR LOCATION SELF-ASSOCIATION INTERACTION WITH BNIP3 AND HUMAN ADENOVIRUS-2 E1B 19 KDA PROTEIN. [5] \" The proapoptotic factor Nix is coexpressed with Bcl-xL during terminal erythroid differentiation. \" Aerbajinai W. Giattina M. Lee Y. T. Raffeld M. Miller J. L. Blood 102:712-717(2003) [PubMed: 12663450] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [6] \" A catalog of genes in the human dermal papilla cells as identified by expressed sequence tags. \" Farooq M. Kim M. K. Kim Y. H. Seo J. M. Lee H. M. Sohn M. Y. Hwang S. Y. Chung H. J. Im S. U. Jung E. J. Kim J. C. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Hair follicle dermal papilla. [7] \" Cloning of human full-length CDSs in BD Creator(TM) system donor vector. \" Kalnine N. Chen X. Rolfs A. Halleck A. Hines L. Eisenstein S. Koundinya M. Raphael J. Moreira D. Kelley T. LaBaer J. Lin Y. Phelan M. Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [8] \" The full-ORF clone resource of the German cDNA consortium. \" Bechtel S. Rosenfelder H. Duda A. Schmidt C. P. Ernst U. Wellenreuther R. Mehrle A. Schuster C. Bahr A. Bloecker H. Heubner D. Hoerlein A. Michel G. Wedler H. Koehrer K. Ottenwaelder B. Poustka A. Wiemann S. Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. [9] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow and Cervix. [10] \" The p53-inducible TSAP6 gene product regulates apoptosis and the cell cycle and interacts with Nix and the Myt1 kinase. \" Passer B. J. Nancy-Portebois V. Amzallag N. Prieur S. Cans C. Roborel de Climens A. Fiucci G. Bouvard V. Tuynder M. Susini L. Morchoisne S. Crible V. Lespagnol A. Dausset J. Oren M. Amson R. Telerman A. Proc. Natl. Acad. Sci. U. S. A. 100:2284-2289(2003) [PubMed: 12606722] [Abstract]Cited for: INTERACTION WITH STEAP3.
Amount: 0.1 mg
Available: In stock
Listprice: €468.97
Price: €468.97
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