Antibodies

MOUSE ANTI AMPK ALPHA 1/2

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against other
Applications WB, IF, IP
Amount 0.1 mg
Host Mouse
ArtNr 20-783-314674
Available
Genway ID:
GWB-CDD88B
Specificity:
AMPK ALPHA 1/2
Isotype:
IgG2aSpecies Cross Reactivity: Reacts with: Vertebrates Human Mouse RatN. B. Antibody reactivity and working conditions may vary between species.
Preparation:
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09% Sodium Azide (NaN3)Approx. Protein Concentrations: IgG concentration 1. 0mg/ml
Immunogen:
29 amino acid peptide within the N-terminal catalytic domain of Drosophila AMPK alpha.
Specificity:
Recognises both the alpha 1 and the alpha 2 isoforms of AMP-activated protein kinase (AMPK). AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit which is stabilised by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia ischemia exercise low glucose levels. AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. Recent studies suggest that AMPK functions as a suppressor of cell proliferation of both malignant and normal cells. Recommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGHuCAL Anti Mouse IgG2aRabbit Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMGoat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L)
Function:
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5\' -AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Ref. 1Catalytic activityATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Enzyme regulationBinding of AMP results in allosteric activation inducing phosphorylation on Thr-172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions without detectable changes in the AMP/ATP ratio By similarity. Subunit structureHeterotrimer of a catalytic subunit a beta and a gamma non-catalytic subunits. Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain. [1] Mural R. J. Istrail S. Sutton G. G. Florea L. Halpern A. L. Mobarry C. M. Lippert R. Walenz B. Shatkay H. Dew I. Miller J. R. Flanigan M. J. Edwards N. J. Bolanos R. Fasulo D. Halldorsson B. V. Hannenhalli S. Turner R. Yooseph S. Lu F. Nusskern D. R. Shue B. C. Zheng X. H. Zhong F. Delcher A. L. Huson D. H. Kravitz S. A. Mouchard L. Reinert K. Remington K. A. Clark A. G. Waterman M. S. Eichler E. E. Adams M. D. Hunkapiller M. W. Myers E. W. Venter J. C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. [2] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [3] \" The interferon-inducible p47 (IRG) GTPases in vertebrates: loss of the cell autonomous resistance mechanism in the human lineage. \" Bekpen C. Hunn J. P. Rohde C. Parvanova I. Guethlein L. Dunn D. M. Glowalla E. Leptin M. Howard J. C. Genome Biol. 6:R92. 1-R92. 18(2005) [PubMed: 16277747] [Abstract]Cited for: IDENTIFICATION INDUCTION. [4] \" Human IRGM induces autophagy to eliminate intracellular mycobacteria. \" Singh S. B. Davis A. S. Taylor G. A. Deretic V. Science 313:1438-1441(2006) [PubMed: 16888103] [Abstract]Cited for: FUNCTION TISSUE SPECIFICITY. [5] \" Sequence variants in the autophagy gene IRGM and multiple other replicating loci contribute to Crohn\' s disease susceptibility. \" Parkes M. Barrett J. C. Prescott N. J. Tremelling M. Anderson C. A. Fisher S. A. Roberts R. G. Nimmo E. R. Cummings F. R. Soars D. Drummond H. Lees C. W. Khawaja S. A. Bagnall R. Burke D. A. Todhunter C. E. Ahmad T. Onnie C. M. McArdle W. Strachan D. Bethel G. Bryan C. Lewis C. M. Deloukas P. Forbes A. Sanderson J. Jewell D. P. Satsangi J. Mansfield J. C. Cardon L. Mathew C. G. Nat. Genet. 39:830-832(2007) [PubMed: 17554261] [Abstract]Cited for: VARIANTS ASP-17 AND LYS-94 TISSUE SPECIFICITY.
Amount: 0.1 mg
Available: In stock
Listprice: €468.97
Price: €468.97
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