MOUSE ANTI AMPK ALPHA 1/2

GWB-CDD88B

IgG2aSpecies Cross Reactivity: Reacts with: Vertebrates Human Mouse RatN. B. Antibody reactivity and working conditions may vary between species.

Phosphate buffered saline pH7. 4

29 amino acid peptide within the N-terminal catalytic domain of Drosophila AMPK alpha.

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5\' -AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Ref. 1Catalytic activityATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Enzyme regulationBinding of AMP results in allosteric activation inducing phosphorylation on Thr-172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions without detectable changes in the AMP/ATP ratio By similarity. Subunit structureHeterotrimer of a catalytic subunit a beta and a gamma non-catalytic subunits. Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain. [1] Mural R. J. Istrail S. Sutton G. G. Florea L. Halpern A. L. Mobarry C. M. Lippert R. Walenz B. Shatkay H. Dew I. Miller J. R. Flanigan M. J. Edwards N. J. Bolanos R. Fasulo D. Halldorsson B. V. Hannenhalli S. Turner R. Yooseph S. Lu F. Nusskern D. R. Shue B. C. Zheng X. H. Zhong F. Delcher A. L. Huson D. H. Kravitz S. A. Mouchard L. Reinert K. Remington K. A. Clark A. G. Waterman M. S. Eichler E. E. Adams M. D. Hunkapiller M. W. Myers E. W. Venter J. C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. [2] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [3] \" The interferon-inducible p47 (IRG) GTPases in vertebrates: loss of the cell autonomous resistance mechanism in the human lineage. \" Bekpen C. Hunn J. P. Rohde C. Parvanova I. Guethlein L. Dunn D. M. Glowalla E. Leptin M. Howard J. C. Genome Biol. 6:R92. 1-R92. 18(2005) [PubMed: 16277747] [Abstract]Cited for: IDENTIFICATION INDUCTION. [4] \" Human IRGM induces autophagy to eliminate intracellular mycobacteria. \" Singh S. B. Davis A. S. Taylor G. A. Deretic V. Science 313:1438-1441(2006) [PubMed: 16888103] [Abstract]Cited for: FUNCTION TISSUE SPECIFICITY. [5] \" Sequence variants in the autophagy gene IRGM and multiple other replicating loci contribute to Crohn\' s disease susceptibility. \" Parkes M. Barrett J. C. Prescott N. J. Tremelling M. Anderson C. A. Fisher S. A. Roberts R. G. Nimmo E. R. Cummings F. R. Soars D. Drummond H. Lees C. W. Khawaja S. A. Bagnall R. Burke D. A. Todhunter C. E. Ahmad T. Onnie C. M. McArdle W. Strachan D. Bethel G. Bryan C. Lewis C. M. Deloukas P. Forbes A. Sanderson J. Jewell D. P. Satsangi J. Mansfield J. C. Cardon L. Mathew C. G. Nat. Genet. 39:830-832(2007) [PubMed: 17554261] [Abstract]Cited for: VARIANTS ASP-17 AND LYS-94 TISSUE SPECIFICITY.