MOUSE ANTI PROSTATE SPECIFIC MEMBRANE ANTIGEN

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Applications WB
Amount 0.1 mg
Host Mouse
ArtNr 20-783-314758
Available
Genway ID:
GWB-7CE422
Specificity:
PROSTATE SPECIFIC MEMBRANE ANTIGEN
Isotype:
IgG1Species Cross Reactivity: Reacts with: Rat PigN. B. Antibody reactivity and working conditions may vary between species.
Preparation:
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09% Sodium Azide (NaN3)Approx. Protein Concentrations: IgG concentration 1. 0mg/ml
Immunogen:
Recombinant fragment corresponding to amino acids 44-750 of human GCPII.
Specificity:
Specifically recognises amino acids 100-104 of the extracellular domain of prostate specific membrane antigen (PSMA) otherwise known as GCPII (glutamate carboxypeptidase II) a 95-110kDa type II transmembrane glycoprotein and member of the peptidase M28 family. Nomenclature for GCPII is dependent upon tissue specificity. PSMA is assigned to GCPII of prostate origin which is up-regulated in prostate cancer and a reliable diagnostic marker. Folate hydrolase (FOLH1) describes GCPII in the intestine and is required for folate uptake whilst NAALADase refers to GCPII expressed in the central nervous system which acts to cleave N-acetylaspartylglutamate (NAAG) to release glutamate implicated in pathological conditions including Alzheimer\' s and Huntington\' s disease. Recommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L)
Function:
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine required for the uptake of folate. In the brain modulates excitatory neurotransmission through the hydrolysis of the neuropeptide N-aceylaspartylglutamate (NAAG) thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro cleaves Gly-Pro-AMC. Catalytic activityRelease of an unsubstituted C-terminal glutamyl residue typically from Ac-Asp-Glu or folylpoly-gamma-glutamates. Cofactor: Binds 2 zinc ions per subunit. Required for NAALADase activity. Ref. 25Enzyme regulationThe NAALADase activity is inhibited by beta-NAAG quisqualic acid 2-(phosphonomethyl) pentanedioic acid (PMPA) and EDTA. Activated by cobalt. Ref. 27Subunit structureHomodimer. Ref. 25Subcellular locationCell membrane; Single-pass type II membrane proteinRef. 16. Isoform PSMA\' : CytoplasmRef. 16. Tissue specificityHighly expressed in prostate epithelium. Detected in urinary bladder kidney testis ovary fallopian tube breast adrenal gland liver esophagus stomach small intestine colon and brain (at protein level). Detected in the small intestine brain kidney liver spleen colon trachea spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA\' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Ref. 3Ref. 9Ref. 23Ref. 24Induction: In the prostate up-regulated in response to androgen deprivation. Ref. 27
Domain:
The NAALADase activity is found in the central region the dipeptidyl peptidase IV type activity in the C-terminal. Ref. 19Post-translational modificationThe first two amino acids at the N-terminus of isoform PSMA\' appear to be cleaved by limited proteolysis. The N-terminus is blocked.
Polymorphism:
Genetic variation in FOLH1 may be associated with low folate levels and consequent hyperhomocysteinemia. This condition can result in increased risk of cardiovascular disease neural tube defects and cognitive deficits.
Miscellaneous:
PSMA is used as a diagnostic and prognostic indicator of prostate cancer and as a possible marker for various neurological disorders such as schizophrenia Alzheimer disease and Huntington disease. Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily. Biophysicochemical properties pH dependence:Stable at pH greater than 6. 5.
Amount: 0.1 mg
Available: In stock
Listprice: €389.48
Discount: -10.0%
Price: €350.53
You save: €38.95
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