MOUSE ANTI PROSTATE SPECIFIC MEMBRANE ANTIGEN

GWB-7CE422

IgG1Species Cross Reactivity: Reacts with: Rat PigN. B. Antibody reactivity and working conditions may vary between species.

Phosphate buffered saline pH7. 4

Recombinant fragment corresponding to amino acids 44-750 of human GCPII.

Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine required for the uptake of folate. In the brain modulates excitatory neurotransmission through the hydrolysis of the neuropeptide N-aceylaspartylglutamate (NAAG) thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro cleaves Gly-Pro-AMC. Catalytic activityRelease of an unsubstituted C-terminal glutamyl residue typically from Ac-Asp-Glu or folylpoly-gamma-glutamates. Cofactor: Binds 2 zinc ions per subunit. Required for NAALADase activity. Ref. 25Enzyme regulationThe NAALADase activity is inhibited by beta-NAAG quisqualic acid 2-(phosphonomethyl) pentanedioic acid (PMPA) and EDTA. Activated by cobalt. Ref. 27Subunit structureHomodimer. Ref. 25Subcellular locationCell membrane; Single-pass type II membrane proteinRef. 16. Isoform PSMA\' : CytoplasmRef. 16. Tissue specificityHighly expressed in prostate epithelium. Detected in urinary bladder kidney testis ovary fallopian tube breast adrenal gland liver esophagus stomach small intestine colon and brain (at protein level). Detected in the small intestine brain kidney liver spleen colon trachea spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA\' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Ref. 3Ref. 9Ref. 23Ref. 24Induction: In the prostate up-regulated in response to androgen deprivation. Ref. 27

Genetic variation in FOLH1 may be associated with low folate levels and consequent hyperhomocysteinemia. This condition can result in increased risk of cardiovascular disease neural tube defects and cognitive deficits.