PCNA

PCNANCBI

PCNA

Rat PCNA made in the protein A expression vector pR1T2T

This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat pre-treatment prior to staining of paraffin sections. Histology: Tonsil

Flow Cytometry - 1/100Immunohistology - Paraffin - 1/50 - 1/100Western Blotting - 1/1000 Summary: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. Summary: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq]. 1. Matthews. M. B. et al. (1984) Identity of the proliferating cell nuclear antigen and cyclin. 2. Ogata. K. et al. (1985) Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies. 3. Garcia. R. L. et al. (1989) Analysis of proliferation grade using anti-PCNA/cyclin monoclonal antibodies in fixed. embedded tissues compared with flow cytometry. 4. Landberg. G. et al. (1990) Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and ki-67