CLATHRIN

CLATHRIN

CHC5. 9

This product is specific for clathrin and stains coated vesicles from various organs and tissues tested e. g. brain mammary gland ovaries. It also reacts with the cell lines HeLa SV-40 and RVF-SMC. Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates forming a coated pit which is essential to endocytosis. It is composed of three polypeptides a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows clathrin to form its unique polyhedral network. In nervous tissue OBT0626 cross-reacts with another coated vesicle protein of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species this antibody may react with a cytokeratin component.

Immunohistology - Frozen - 1/10 - 1/50Western Blotting - 1/50 - 1/500