CD49d

CD49dNCBI

CD49d

This product recognises murine alpha 4 integrin (CD49d) a 150kD glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively. CD49d is expressed on most lymphocytes granulocytes monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1) fibronectin and MAdCAM-1. Clone PS/2 has also been reported to block the binding of CD49d to its ligands (3). We recommend the use of for this purpose.

Phosphate buffered saline pH7. 4

Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested

Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

Membrane; Single-pass type I membrane protein.

Phosphorylation on Ser-1027 inhibits PXN binding.

Contains 7 FG-GAP repeats. 1. Miyake. K. et al. (1991) Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis. 2. Miyake. K. et al. (1991) A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture. 3. Andrew. D. P. et al. (1994) Distinct but Overlapping Epitopes Are Involved in alpha4 beta7-Mediated Adhesion to Vascular Cell Adhesion Molecule-1. Mucosal Addressin-1. Fibronectin. and Lymphocyte Aggregation.