Goat anti Rat IgG (H&amp,L)

GWB-838E81

IgG (H& L)

Polyclonal Antibodies to Animal Immunoglobulins

10mM Phosphate pH 8. 0 containing 0. 15M Sodium chlorideApplications Notes : Suitable for use in ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Protocols from PublicationsCitation: 1: Vuilleumier N Rossier MF Pagano S Python M Charbonney E Nkoulou R James R Reber G Mach F Roux-Lombard P. Anti-apolipoprotein A-1 IgG as an independent cardiovascular prognostic marker affecting basal heart rate in myocardial infarction. Eur Heart J. 2010 Apr; 31(7):815-23. Epub 2010 Feb 22. PubMed PMID: 20176799. Species: RatExperiment Name: Cell culture and cell contraction frequencyExperiment Name: Cell culture and cell contraction frequencyExperiment Background: Aim of this experiment was To assess the prognostic value of anti-apolipoprotein A-1 (anti-apoA-1) IgG after myocardial infarction (MI) and its association with major cardiovascular events (MACEs) at 12 months and to determine their association with resting heart rate (RHR) a well-established prognostic feature after MI. Anti-apoA-1 IgG have been reported in MI without autoimmune disease but their clinical significance remains undetermined. Experimental Steps: Neonatal cardiac cells were isolated from 1- to 2-day-old Wistar rat ventricles by digestion with low trypsin-EDTA and type 2 collagenase. Briefly animals were killed (in conformity with the Guide for the Care and use of Laboratory Animals published by the NIH and with the authorization of the local County Veterinary Office) and freshly isolated cells were seeded in plastic flasks to allowselective adhesion of cardiac fibroblasts. Thereafter cardiomyocytes were decanted from the flasks and distributed in laminincoated 90-mm Petri dishes or in 6-well culture plates. Cells from a same preparation were used for testing the various experimental conditions. Spontaneously contracting cell monolayers were incubated for the indicated times with a 10% dilution of MI patients plasma or reference plasma spiked with different concentrations (1?20 mg/mL) of mouse monoclonal anti-human apoA-1 IgG (Abcam Nottingham UK) and respective IgG controls (polyclonals) of the same genetic background ( Life Science Cincinnati OH USA) in serum-free DMEM. Ultra-pure lipid low apoA-1 was provided by R. James and intravenous immunoglobulins (IVIG; EndobulinTM) were purchased from Baxter (Switzerland Volketswil). Cell beating frequency was determined by counting under light microscope the number of contractions per time unit in three different locations of the dish. Those tests were performed after 24 h pre-incubation of cells in serum-free medium with and without aldosterone (10 nM) by an observer blinded to anti-apoA-1 IgG sample status. Results are expressed in beat per minute (b. p. m. ). Experiments were also performed with addition of antibodies directly into serum-free culture medium.

Secreted.

Nie has the G1M(17) allotypic marker 97-K and the G1M(1) markers 239-D and 241-L. KOL and EU sequences have the G1M(3) marker and the G1M (non-1) markers.

EU also differs in the amidation states of residues 155 166 177 195 198 269 and 272 and in the order of residues 268-272.