SHEEP ANTI RABBIT PHOSPHORYLASE B

PHOSPHORYLASE B

Purified IgG prepared by affinity chromatography on Protein G

Recognises phosphorylase B a catalytic protein with a predicted molecular weight of 125 kDa localized in the cytoplasm and cell membrane. In skeletal muscle phosphorylase occurs in two forms phosphorylase A and phosphorylase B. Phosphorylase A is the more active of the two forms and has a higher molecular weight. The two forms are interconvertable. Phosphorylase B catalyzes the phosphorylation of serine residues in various substrates including troponin I. 7370-9808G shows high cross reactivity (> 95%) with phosphorylase A in ELISA. Recommended Secondary Antibodies: Rabbit Anti Sheep IgG (H/L)Donkey Anti Sheep IgG

Glycan biosynthesis; glycogen metabolism. Subunit structurePolymer of 16 chains four each of alpha beta gamma and delta. Alpha and beta are regulatory chains gamma is the catalytic chain and delta is calmodulin. Subcellular locationCell membrane; Lipid-anchor; Cytoplasmic side Potential. Post-translational modificationCys-1090 is farnesylated but the terminal tripeptide is not removed and the cysteine carboxyl is not methylated. Sequence similaritiesBelongs to the phosphorylase b kinase regulatory chain family. 1. Yoshino N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49: 97 - 110. 2. Kim C. et al. (2002) Trafficking machinery of NKT cells: shared and differential chemokine receptor expression among V alpha 24+ V beta 11+ NKT cell subsets with distinct cytokine - producing capacity. Blood 100: 11 - 16. [1] \" Cloning of interleukin 2 mRNAs from human tonsils. \" Maeda S. Nishino N. Obaru K. Mita S. Nomiyama H. Shimada K. Fujimoto K. Teranishi T. Hirano T. Onoue K. Biochem. Biophys. Res. Commun. 115:1040-1047(1983) [PubMed: 6312994] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [2] \" Structure and expression of a cloned cDNA for human interleukin-2. \" Taniguchi T. Matsui H. Fujita T. Takaoka C. Kashima N. Yoshimoto R. Hamuro J. Nature 302:305-310(1983) [PubMed: 6403867] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. [3] \" Molecular cloning of human interleukin 2 cDNA and its expression in E. coli. \" Devos R. Plaetinck G. Cheroutre H. Simons G. Degrave W. Tavernier J. Remaut E. Fiers W. Nucleic Acids Res. 11:4307-4323(1983) [PubMed: 6306584] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [4] \" Structure of the human interleukin 2 gene. \" Fujita T. Takaoka C. Matsui H. Taniguchi T. Proc. Natl. Acad. Sci. U. S. A. 80:7437-7441(1983) [PubMed: 6324170] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [5] \" DNA sequence of the 5\' flanking region of the human interleukin 2 gene: homologies with adult T-cell leukemia virus. \" Holbrook N. J. Lieber M. Crabtree G. R. Nucleic Acids Res. 12:5005-5013(1984) [PubMed: 6330695] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [6] \" T-cell growth factor: complete nucleotide sequence and organization of the gene in normal and malignant cells. \" Holbrook N. J. Smith K. A. Fornace A. J. Jr. Comeau C. M. Wiskocil R. L. Crabtree G. R. Proc. Natl. Acad. Sci. U. S. A. 81:1634-1638(1984) [PubMed: 6608729] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [7] \" Interleukin-2 transcripts in human and rodent brains: possible expression by astrocytes. \" Eizenberg O. Faber-Elman A. Lotan M. Schwartz M. J. Neurochem. 64:1928-1936(1995) [PubMed: 7722480] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [8] \" Sequence of interleukin-2 isolated from human placental poly A+ RNA: possible role in maintenance of fetal allograft. \" Chernicky C. L. Tan H. Burfeind P. Ilan J. Ilan J. Mol. Reprod. Dev. 43:180-186(1996) [PubMed: 8824916] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta. [9] SeattleSNPs variation discovery resourceSubmitted (MAR-2001) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [10] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood.