Human Growth Hormone (HGH) Enzyme Immunoassay Test Kit

Human Growth Hormone (HGH)Sample Type: Serum# of Samples: 1X96 Assays
Intended Use: The Biotech HGH ELISA is intended for the quantitative determination of human growth hormone (HGH) concentration in human serum. Principles of the assay: The Biotech HGH ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay (ELISA). The assay system utilizes a sheep anti-HGH antibody for solid phase (microtiter wells) immobilization and a mouse monoclonal anti-HGH antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the antibodies resulting in HGH molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 45 minute incubation at room temperature the wells are washed with water to remove unbound labeled antibodies. A solution of TMB is added and incubated for 20 minutes resulting in the development of a blue color. The color development is stopped with the addition of 1N HCl and the color is changed to yellow and measured spectrophotometrically at 450 nm. The concentration of HGH is directly proportional to the color intensity of the test sample. Summary and Explanation of Test: Limitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with good laboratory practice and adherence to the package insert instructions. 2. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Secreted.

Defects in GH1 are the cause of Kowarski syndrome [MIM:262650]; also known as pituitary dwarfism VI.

Defects in GH1 may be a cause of short stature [MIM:604271]. Short stature is defined by a subnormal rate of growth. Pharmaceutical: Available under the names Nutropin or Protropin (Genentech) Norditropin (Novo Nordisk) Genotropin (Pharmacia Upjohn) Humatrope (Eli Lilly) and Saizen or Serostim (Serono). Used for the treatment of growth hormone deficiency and for Turner\' s syndrome.

Belongs to the somatotropin/prolactin family.