Human Leptin ELISA

Human Leptin
ELISA# of Samples: 1X96 Assays
Intended Use: The Human Leptin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human leptin in serum plasma and tissue culture medium. It is intended for reserach use only. Principle of the test: In \' s Human Leptin ELISA standards quality controls and samples of sera are incubated in microtitration wells coated with anti-human leptin antibody. After a thorough wash anti-human leptin antibody labelled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody-leptin complex. Following another washing step the remaining HRP-conjugated antibody is allowed to react with the substrate tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured spectrophotometrically at 450 nm. The absorbance is proportional to the concentration of leptin. A standard curve is constructed by plotting absorbance values versus leptin concentrations of standards and concentrations of unknown samples are determined using this standard curve. Storage and Stability: Store the kit at 2-8C. Under these conditions the kit is stable until the expiration date (see label on the box). Limitation of the Test: Results exceeding 50 ng/ml should be repeated with more diluted sample (e. g. 1:5). Dilution factors need to be taken into consideration in calculating the concentration of leptin. Sensitivity and

Human Leptin ELISA

Interacts with SIGLEC6.

Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].