IGG Antibodies to Rubella Virus ELISA Quantitation Kit

IGG Antibodies to Rubella VirusSample Type: Serum# of Samples: 1X96 Assays
Intended Use: The Biotech Rubella IgG ELISA is intended for use in evaluating serologic status to the rubella virus infection. It is also used to evaluate paired sera for the presence of a significant increase in specific Rubella IgG as indication of a recent or current rubella virus infection. Introduction: Rubella is a herpes virus. Generally rubella is considered a mild adolescent disease. However a maternal infection can be transmitted through the placenta to the fetus causing congenital rubella. Congenital rubella may result in chronic cardiac disease growth retardation hepatosplenomegaly malformations and other severe anomalies. Children born asymptomatic may develop these abnormalities later in life. To reduce the risk of such severe complications accurate serological methods must be performed to determine the serologic status of childbearing-aged women. The presence of rubella- specific IgG in the bloodstream attests immunity to rubella. A woman tested to be non-immune can be educated on infection and differentiate rubella from other exanthematous diseases. Expecting women with current rubella infection should be counseled on the consequences of congenital infection. Principle of the test: Purified Rubella antigen is coated on the surface of microwells. Diluted serum is added to the wells and the Rubella IgG- specific antibody if present binds to the antigen during incubation. After washing the wells to remove unbound sample antibody to human IgG conjugated with horseradish peroxidase (HRP) is added and incubated at 37C for 30 minutes. Unbound conjugate is removed by a subsequent washing step. A solution of TMB Reagent is then added to the microwells. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrators and controls. Limitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice. 2. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings. 3. Serum samples demonstrating gross lipemia gross hemolysis or turbidity should not be used with this test. Precision: A study was performed to document typical assay precision with the Biotech Rubella IgG EIA product. The mean SD and %CV were calculated for Intra- and Inter-Assay in concentration value (IU/mL).

Secreted.

Nie has the G1M(17) allotypic marker 97-K and the G1M(1) markers 239-D and 241-L. KOL and EU sequences have the G1M(3) marker and the G1M (non-1) markers.

EU also differs in the amidation states of residues 155 166 177 195 198 269 and 272 and in the order of residues 268-272.