IGM Antibodies to Rubella Virus ELISA Quantitation Kit

IGM Antibodies to Rubella VirusSample Type: Serum# of Samples: 1X96 Assays
Intended Use: The Rubella IgM ELISA is intended for use in the detection of IgM antibody to the rubella virusIntroduction: Rubella is a herpes virus. Generally rubella is considered a mild adolescent disease. However a maternal infection can be transmitted through the placenta to the fetus causing congenital rubella. Congenital rubella may result in chronic cardiac disease growth retardation hepatosplenomegaly malformations and other severe anomalies. Children born asymptomatic may develop these abnormalities later in life. To reduce the risk of such severe complications accurate serological methods must be performed to determine the serologic status of childbearing-aged women. A woman tested to be non-immune can be educated on infection and differentiate rubella from other exanthematous diseases. Expecting women with current rubella infection should be counseled on the consequences of congenital infection. Principle of the test: Purified Rubella antigen is coated on the surface of microwells. Diluted serum is added to the wells and the Rubella IgM- specific antibody if present binds to the antigen during incubation. After washing the wells to remove unbound sample antibody to human IgM conjugated with horseradish peroxidase (HRP) is added and incubated at 37C for 30 minutes. Unbound conjugate is removed by a subsequent washing step. A solution of TMB Reagent is then added to the microwells. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls. Limitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice. 2. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings. 3. Serum samples demonstrating gross lipemia gross hemolysis or turbidity should not be used with this test. Precision: A study was performed to document typical assay precision with the Biotech Rubella IgM EIA product. The mean SD and %CV were calculated for Intra- and Inter-Assay in O. D. value (A450).

Isoform 1: Secreted. Note=During differentiation B-lymphocytes switch from expression of membrane-bound IgM to secretion of IgM.

All 4 combinations of the S/G and V/G polymorphisms at positions 191 and 216 have been observed in human mu chains.