Insulin ELISA Kit

GWB-D9BD0E

Diabetes & amp; MetabolismSample Type: Serum and plasmaSensitivity: 0 15 uIU/mLDescription: Immunoenzymetric assay for the in vitro quantitative measurement of human Insulin (INS) in serum and plasma.
Intended Use: Immunoenzymetric assay for research quantitative measurement of human Insulin (INS) in serum. Background: A Biological activities of insulinInsulin a polypeptide hormone with a molecular weight of 5800 Da is secreted by the beta cells of the islets of Langerhans from the pancreas. Insulin possesses a wide spectrum of biological actions. It stimulates cellular glucose uptake glucose oxidation glycogenesis lipogenesis proteogenesis and the formation of DNA and RNA. Insulin plays a key role in the regulation of plasma glucose levels (hepatic output inhibition stimulation of peripheral glucose utilisation). The resulting hypoglycemic effects of insulin are counterbalanced by hormones with hyperglycemic effects (glucagon growth hormone cortisol epinephrin). Insulin secretion is mainly controlled by the plasma glucose levels : hyperglycemia induces a prompt and important increase in circulating insulin levels. Neural influences as well as various metabolic and hormonal factors (amino acids glucagon gastro intestinal hormone) also participate to the control of insulin secretion. Type I (insulin dependent : \" juvenile\" ) diabetes is due to a destruction of the beta cells with a consequence of absolute lack of insulin. In type II (non-insulin-dependent : \" maturity onset\" ) diabetes insulin resistance may play an important role; however after several years of evolution beta-cells failure may occur leading to a relative insulinopenia requiring in some cases insulin administration. Insulin resistance is associated with high circulation levels of the hormone. The most common case of insulin resistance is represented by obesity. Various endocrinopathies (acromegaly Cushing syndrome) as well as rare cases of insulin receptor defects or cases with anti-insulin receptor antibodies are associated with glucose intolerance or even diabetes due to insulin resistance. The determination of plasma insulin levels is an important parameter in the diagnosis of hypoglycemia. Insulin levels are high in cases of insulinoma (beta-cell tumor). Functional postprandial hypoglycemia may also be associated with inappropriate insulin release to carbohydrate intake. Insulin levels are determined either in the fasting state or during dynamic test :a) stimulation test : carbohydrate rich meal oral glucose tolerance test (OGTT) arginin infusion tolbutamide or other sulfonylureas administration. b) inhibition test : fasting somatostatine infusionPrinciples of the method: The INS-EASIA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on breakable microtiterplates. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of insulin. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 - human insulin - MAb 2 - HRP the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance which is proportional to the insulin concentrationA calibration curve is plotted and INS concentration in samples is determined by interpolation from the calibration curve. Storage and Stability: ? Before opening or reconstitution all kits components are stable until the expiry date indicated on the vial label if kept at 2 to 8C. ? Unused wells must be stored at 2-8C in a sealed bag containing a desiccant until expiration date. ? After reconstitution calibrators and controls are stable for 1 week at 2 to 8C. For longer storage periods aliquots should be made and kept at -20C. Avoid successive freeze thaw cycles. ? The concentrated Wash Solution is stable at room temperature until expiration date. ? Freshly prepared Working Wash solution should be used on the same day. ? After its first use the conjugate is stable until expiry date if kept in the original well-closed vial at 2 to 8C. ? Alterations in physical appearance of kit reagents may indicate instability or deterioration.

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides amino acids and fatty acids. It accelerates glycolysis the pentose phosphate cycle and glycogen synthesis in liver.

Secreted.

Belongs to the insulin family.