Estrone ELISA Kit

GWB-F11E46

FertilitySample Type: Serum and plasmaSensitivity: 6 3 pg/mLDescription: Estrone Enzyme Immunoassay Kit provides materials for the Quantitative Determination of Estrone in serum and plasmaIntroduction: The Estrone Enzyme Immunoassay Kit provides materials for the quantitative determination of Estrone in serum and plasma. This assay is intended for reserach use only. Estrone (3-hydroxy-1 3 5 (10)-estratrien-17-one) is beside estradiol and estriol one of the three major naturally occuring estrogens. The estrogens are involved in the development of female sex organs and secondary sex characteristics. Bioassay data indicate that the estrogenic activity of estrone is considerably lower in comparison to estradiol (1). However the physiological role of endogenous estrone is not well defined. Estrone is produced primarily from androstenedione. In premenopausal women more than 50% of the estrone is secreted by the ovary. In prepubertal children men and postmenopausal women the major portion of estrone is derived from peripheral tissue conversion (2). During the follicular phase of the menstrual cycle the estrone level increases with a clear peak around day 13. The peak is of short duration and by day 16 of the cycle levels will be low again. A second peak during the luteal phase occurs around day 21 of the cycle. If fertilization does not occur production of estrone decreases again. These changes of estrone concentration are in parallel to that of estradiol (3). Until the 4. to 6. week of pregnancy estrone originates primarily from maternal sources such as the ovaries adrenals or peripheral conversion thus remaining within the normal values (4) . After the 6. to 10. week of pregnancy the values increase gradually due to placental secretion of estrone. After menopause estrone levels do not decline as dramatically as estradiol levels. In postmenopausal women estrone is the major estrogen. In males the concentration of E1 has been reported to rise up with age inversely to that of 17-OH-progesterone (5). In premenopausal women excessive estrone levels can result from the conversion of large amounts of androstenedione produced in polycystic ovary syndrom (6) and ovarian tumors. Principle of the test: The Estrone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the principle of competitive binding. The microtiter wells are coated with an antibody directed towards an antigenic site on the Estrone molecule. Endogenous Estrone of a patient sample competes with an Estrone horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of Estrone in the sample. After addition of the substrate solution the intensity of colour developed is reverse proportional to the concentration of Estrone in the patient sample. Storage and Stability: When stored at 2 to 8C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date. All opened reagents must be stored at 2 to 8C. Microtiter wells must be stored at 2 to 8C. Once the foil bag has been opened care should be taken to close it tightly again.

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs.

Endoplasmic reticulum lumen. Note=Microsomal membrane lumen of endoplasmic reticulum.