HDAC developer (2X)

HDAC developer (2X)Product Type: Reagent--Kit componentProduct Description: HDAC assay developer is an essential reagent for signal generation in HDACor sirtuin assays done with any of HDAC fluorimetric substrates An appropriate fluorimetric substrate comprising an acetylated lysine side chain isfirst incubated with a sample containing HDAC activity nuclear or cellular extract purifiedenzyme bead-bound immunocomplex etc. Deacetylation of the substrate sensitizes it so that in a second step treatment with the HDAC assay developer produces a fluorophore. Typically an ?inhibitor stop\' will be included in the HDAC developer e. g. Trichostatin A for class I and IIHDACs nicotinamide for sirtuins .