RECOMBINANT HUMAN CD95 (SOLUBLE)

CD95 (SOLUBLE)Species Cross Reactivity: Reacts with: MouseN. B. Antibody reactivity and working conditions may vary between species.

Phosphate buffered saline

CD95 also known as Fas/APO-1 is a death-domain (DD) containing type I membrane glycoprotein and member of the TNF receptor superfamily expressed by activated T and B cells neutrophils monocytes and thymocytes which binds to its ligand CD95L (FasL/CD178) to mediate apoptosis. The soluble form of CD95 (sCD95) generated by alternative splicing of CD95 mRNA has the ability to inhibit target cell apoptosis by neutralizing CD95L. Elevated levels of sCD95 have been detected in patients with systemic lupus erythematosus (SLE) and have been associated with a poor prognosis in renal cell cancer and melanoma patients. PHP192 can be used in functional studies to inhibit sCD95/CD95L mediated lysis of CD95 sensitive cells. 1. Schmid S. L. (1997) Clathrin-coated vesicle formation and protein sorting: An integrated process. Annu. Rev. Biochem. 66: 511-548. [1] \" Structure and expression of a human gene coding for a 71 kd heat shock \' cognate\' protein. \" Dworniczak B. P. Mirault M. -E. Nucleic Acids Res. 15:5181-5197(1987) [PubMed: 3037489] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1). [2] \" Molecular and functional characterization of HSC54 a novel variant of human heat shock cognate protein 70. \" Tsukahara F. Yoshioka T. Muraki T. Mol. Pharmacol. 58:1257-1263(2000) [PubMed: 11093761] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). [3] \" Complete coding sequence of human HSC70. \" Niswonger M. L. Berk L. R. Srivastava P. K. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). [4] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta and Skin. [5] Bienvenut W. V. Waridel P. Quadroni M. Submitted (MAR-2009) to UniProtKBCited for: PROTEIN SEQUENCE OF 2-49; 57-71; 77-102; 103-155; 160-188; 221-247; 273-311; 326-342; 349-416; 424-447; 452-493; 510-517; 540-550; 570-597 AND 602-646 CLEAVAGE OF INITIATOR METHIONINE ACETYLATION AT SER-2 MASS SPECTROMETRY. Tissue: Embryonic kidney. [6] Bienvenut W. V. Lilla S. von Kriegsheim A. Lempens A. Kolch W. Bilsland A. E. Keith W. N. Submitted (JAN-2010) to UniProtKBCited for: PROTEIN SEQUENCE OF 2-49; 57-72; 78-88; 113-188; 221-247; 300-319; 326-342; 349-357; 362-384; 424-447; 452-469; 510-517; 540-550 AND 584-609 CLEAVAGE OF INITIATOR METHIONINE ACETYLATION AT SER-2 MASS SPECTROMETRY. Tissue: Colon carcinoma and Ovarian carcinoma. [7] Lubec G. Vishwanath V. Chen W. -Q. Sun Y. Submitted (DEC-2008) to UniProtKBCited for: PROTEIN SEQUENCE OF 4-49; 57-71; 77-88; 113-126; 129-155; 160-187; 221-246; 300-311; 329-342; 362-384; 424-447; 540-550 AND 574-583 MASS SPECTROMETRY. Tissue: Brain Cajal-Retzius cell and Fetal brain cortex. [8] \" Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes. \" Rasmussen H. H. van Damme J. Puype M. Gesser B. Celis J. E. Vandekerckhove J. Electrophoresis 13:960-969(1992) [PubMed: 1286667] [Abstract]Cited for: PROTEIN SEQUENCE OF 50-55; 103-107 AND 580-596. [9] \" Identification of the 70kD heat shock cognate protein (Hsc70) and alpha-actinin-1 as novel phosphotyrosine-containing proteins in T lymphocytes. \" Egerton M. Moritz R. L. Druker B. Kelso A. Simpson R. J. Biochem. Biophys. Res. Commun. 224:666-674(1996) [PubMed: 8713105] [Abstract]Cited for: PROTEIN SEQUENCE OF 77-86; 221-236 AND 302-311. [10] \" Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells. \" Hattori H. Liu Y. -C. Tohnai I. Ueda M. Kaneda T. Kobayashi T. Tanabe K. Ohtsuka K. Cell Struct. Funct. 17:77-86(1992) [PubMed: 1586970] [Abstract]Cited for: SUBCELLULAR LOCATION.